Experimental Conditions for Mapping Nanomechanical Properties of Live Cells with AFM
My question is in regards to the paper by Raman et al published in Nature Nanotechnology in 2011 (DOI: 10.1038/NANO.2011.186).
How does driving the cantilever in the repulsive or attractive regimes (instead of exactly at the resonant frequency) effect calculations of stiffness and damping?
Can the procedure outlined in the paper be applied to cells in media? Especially with a large concentration of serum?
Thank you in advance