BME 695N Lecture 17: Assessing nanotoxicity at the single cell level

06 Nov 2007 | Online Presentations | Contributor(s): James Leary

Outline: Outline – the need for single cell measures of nanotoxicity There is more than one way for a cell to die... Necrosis" vs. "Apoptosis" There are other forms of "toxicity" Some other challenges in measuring toxicity of nanomaterials Necrosis vs. Apoptosis …

BME 695N Lecture 16: Assessing drug efficacy at the single cell level

02 Nov 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction and overview Nanomedical treatment at the single cell level requires evaluation at the single cell level For evaluation purposes, does structure reveal function? The difficulty of anything but simple functional assays The need for assays which at least show correlation to …

BME 695N Lecture 15: Nanodelivery of therapeutic genes & molecular biosensor feedback control systems

30 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction and overview Some of the advantages of therapeutic genes Some of the advantages of molecular biosensor feedback control systems Why a nanodelivery approach is appropriate The therapeutic gene approach What constitutes a "therapeutic gene" ? Transient versus stable …

BME 695N Lecture 14: Challenges of proper drug dosing with nanodelivery systems

29 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Overview of drug dosing problem Problems of scaling up doses from animal systems Basing dosing on size, area, weight of recipient Vast differences between adults in terms of genetics, metabolism Dosing in children – children are NOT smaller adults! Pharmacokinetics – drug …

BME 695N Lecture 13: Assessing Zeta Potentials

29 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction – the importance of the zeta potential Nanoparticle-nanoparticle interactions Nanoparticle-cell interactions Part of the initial nanomedical system-cell targeting process Low zeta potential leads to low serum protein binding and potentially longer circulation Zeta …

KIST/PU Multi-Component, Multi-Functional Nanomedical Systems for Drug/Gene Delivery

23 Oct 2007 | Online Presentations | Contributor(s): James Leary

In this brief paper we describe some of our recent efforts to construct multi-component, multi-functional nanomedical systems for delivery of therapeutic genes. We first describe the general philosophy of our approach. Then we describe three specific aspects of the overall construction in simple …

BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

26 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Assessing nanomedical system (NMS) targeting at the single cell level Fluorescent labeling of NMSs First estimates of NMS binding by fluorescence microscopy Internal of external binding by confocal microscopy Single-cell image/confocal analysis Flow cytometric quantitation of NMS …

BME 695N Lecture 10: Nanomaterials for core design

26 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction Core building blocks Functional cores Functionalizing the core surface Ferric oxide cores Paramagnetic cores Superparamagnetic cores Ferric nanorods Advantages and disadvantages C60 and carbon nanotubes Size and structure of C60 Elongation of C60 into …

BME 695N Lecture 8: Technologies for measuring nanomedical systems on/within cells

24 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction to measuring technologies for nanomedical system interaction with cells The importance of quantitative or at least semi-quantitative single cell measurements to detect presence and location of nanomedical systems Below "optical limit" imaging Requirements on the NMS to …

BME 695N Lecture 7: Normal & facilitated cell entry mechanisms

15 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction The general problem of cell entry Choosing modes of cell entry How does Nature do it? (biomimetics) Non-specific uptake mechanisms Pinocytosis by all cells Phagocytosis by some cells Receptor mediated uptake Receptor mediated transport of desired …

BME 695N Lecture 5: Cell Targeting

12 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Overview: targeting nanosystems to cells Antibody targeting Peptide targeting Aptamer targeting Antibodies – polyclonal and monoclonal Where do antibodies come from – in nature? How do we make them in the laboratory? Monoclonal antibodies Therapy problems with mouse …

BME 695N Lecture 4: Designing "Theragnostic" Systems

04 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Bridging the gap between diagnostics and therapeutics How conventional medicine is practiced in terms of diagnostics and therapeutics The consequences of separating diagnostics and therapeutics A new approach – "theragnostics" (or "theranostics") Examples of current theragnostic …

BME 695N Lecture 3: Overview of Basic Nanomedical Systems Design

29 Aug 2007 | Online Presentations | Contributor(s): James Leary

Outline: Nanomedical systems – levels of challenges Essential elements of a nanomedical system Requirements for specific cell targeting Consequences of mis-targeting Engineering around the consequences of mis-targeting Some ways to lower mis-targeting to non-diseased cells

BME 695N Lecture 2: Basic Concepts of Nanomedical Systems

28 Aug 2007 | Online Presentations | Contributor(s): James Leary

Outline: Features of Nanomedicine Bottoms up rather than top down approach to medicine Nano-tools on the scale of molecules Cell-by-cell repair approach – regenerative medicine Feedback control system to control drug dosing Elements of good engineering design Whenever possible, use …

BME 695N Lecture 1: Need for New Perspectives on Medicine

03 Aug 2007 | Online Presentations | Contributor(s): James Leary

Outline: The Progression of Medicine Conventional "modern" medicine "Personalized" or "molecular" medicine Nanomedicine "single-cell" medicine How Conventional Medicine Works for Diagnosis of Disease Identification of the "diseased state" Simple measurements of body structure and …

Nanotechnologies, Science and Society: Promises and Challenges

10 May 2007 | Online Presentations | Contributor(s): James Leary

James Leary (2007), "Nanotechnologies, Science and Society: Promises and Challenges," Purdue Bioethics Seminar Series, http://nanohub.org/resources/2691. Bioethics at Purdue The Graduate School of Purdue University - Puskas Fellowship

Engineering Nanomedical Systems

06 Mar 2006 | Online Presentations | Contributor(s): James Leary

This tutorial discusses general problems and approaches to the design of engineered nanomedical systems. One example given is the engineering design of programmable multilayered nanoparticles (PMNP) to control a multi-sequence process of targeting to rare cells in-vivo, re-targeting to …

Nanofactories - In - Situ Production of Therapeutic Genes...

27 Jul 2005 | Online Presentations | Contributor(s): James Leary

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