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BME 695N Lecture 20: GMP and issues of quality control manufacture of nanodelivery systems
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15 Nov 2007 | Online Presentations | Contributor(s): James Leary
Outline:Overview What does cGMP mean? Why GMP? Controlling processes means more predictable outcomes… Enforcement What can be learned from the semi-conductor industry clean-room and manufacturing? What doesn’t fit this paradigm?cGMP-level manufacturing Predictable methods lead to predictable...
BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use
12 Nov 2007 | Online Presentations | Contributor(s): James Leary
Outline:Overview – the in-vitro to ex-vivo to in-vivo paradigm In-vitro - importance of choosing suitable cell lines Ex-vivo – adding the complexity of in-vivo background while keeping the simplicity of in-vitro In-vivo - all the complexity of ex-vivo plus the “active” components of a real...
BME 695N Lecture 17: Assessing nanotoxicity at the single cell level
06 Nov 2007 | Online Presentations | Contributor(s): James Leary
Outline:Outline – the need for single cell measures of nanotoxicity There is more than one way for a cell to die... Necrosis" vs. "Apoptosis" There are other forms of "toxicity" Some other challenges in measuring toxicity of nanomaterialsNecrosis vs. Apoptosis mechanisms Necrosis is unplanned...
BME 695N Lecture 16: Assessing drug efficacy at the single cell level
02 Nov 2007 | Online Presentations | Contributor(s): James Leary
Outline:Introduction and overviewNanomedical treatment at the single cell level requires evaluation at the single cell levelFor evaluation purposes, does structure reveal function?The difficulty of anything but simple functional assaysThe need for assays which at least show correlation to...
BME 695N Lecture 15: Nanodelivery of therapeutic genes & molecular biosensor feedback control systems
30 Oct 2007 | Online Presentations | Contributor(s): James Leary
Outline:Introduction and overviewSome of the advantages of therapeutic genesSome of the advantages of molecular biosensor feedback control systemsWhy a nanodelivery approach is appropriateThe therapeutic gene approachWhat constitutes a "therapeutic gene" ?Transient versus stable expression...
BME 695N Lecture 14: Challenges of proper drug dosing with nanodelivery systems
29 Oct 2007 | Online Presentations | Contributor(s): James Leary
Outline:Overview of drug dosing problemProblems of scaling up doses from animal systemsBasing dosing on size, area, weight of recipientVast differences between adults in terms of genetics, metabolismDosing in children – children are NOT smaller adults!Pharmacokinetics – drug distribution,...
BME 695N Lecture 13: Assessing Zeta Potentials
Outline:Introduction – the importance of the zeta potentialNanoparticle-nanoparticle interactionsNanoparticle-cell interactionsPart of the initial nanomedical system-cell targeting processLow zeta potential leads to low serum protein binding and potentially longer circulationZeta potential...
KIST/PU Multi-Component, Multi-Functional Nanomedical Systems for Drug/Gene Delivery
23 Oct 2007 | Online Presentations | Contributor(s): James Leary
In this brief paper we describe some of our recent efforts to construct multi-component, multi-functional nanomedical systems for delivery of therapeutic genes. We first describe the general philosophy of our approach. Then we describe three specific aspects of the overall construction in simple...
BME 695N Lecture 10: Nanomaterials for core design
26 Sep 2007 | Online Presentations | Contributor(s): James Leary
Outline:IntroductionCore building blocksFunctional coresFunctionalizing the core surfaceFerric oxide coresParamagnetic coresSuperparamagnetic coresFerric nanorodsAdvantages and disadvantagesC60 and carbon nanotubesSize and structure of C60Elongation of C60 into carbon nanotubesAdvantages and...
BME 695N Lecture 8: Technologies for measuring nanomedical systems on/within cells
24 Sep 2007 | Online Presentations | Contributor(s): James Leary
Outline:Introduction to measuring technologies for nanomedical system interaction with cellsThe importance of quantitative or at least semi-quantitative single cell measurementsto detect presence and location of nanomedical systemsBelow "optical limit" imagingRequirements on the NMS to have...
BME 695N Lecture 7: Normal & facilitated cell entry mechanisms
15 Sep 2007 | Online Presentations | Contributor(s): James Leary
Outline:IntroductionThe general problem of cell entryChoosing modes of cell entryHow does Nature do it? (biomimetics)Non-specific uptake mechanismsPinocytosis by all cellsPhagocytosis by some cellsReceptor mediated uptakeReceptor mediated transport of desired moleculesExample- transferrin...
BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo
Outline:Assessing nanomedical system (NMS) targeting at the single cell levelFluorescent labeling of NMSsFirst estimates of NMS binding by fluorescence microscopyInternal of external binding by confocal microscopySingle-cell image/confocal analysisFlow cytometric quantitation of NMS binding to...
BME 695N Lecture 5: Cell Targeting
12 Sep 2007 | Online Presentations | Contributor(s): James Leary
Outline:Overview: targeting nanosystems to cellsAntibody targetingPeptide targetingAptamer targetingAntibodies – polyclonal and monoclonalWhere do antibodies come from – in nature?How do we make them in the laboratory?Monoclonal antibodiesTherapy problems with mouse monoclonal...
BME 695N Lecture 4: Designing "Theragnostic" Systems
04 Sep 2007 | Online Presentations | Contributor(s): James Leary
Outline:Bridging the gap between diagnostics and therapeuticsHow conventional medicine is practiced in terms of diagnostics and therapeuticsThe consequences of separating diagnostics and therapeuticsA new approach – "theragnostics" (or "theranostics")Examples of current theragnostic...
BME 695N Lecture 3: Overview of Basic Nanomedical Systems Design
29 Aug 2007 | Online Presentations | Contributor(s): James Leary
Outline:Nanomedical systems – levels of challengesEssential elements of a nanomedical systemRequirements for specific cell targetingConsequences of mis-targetingEngineering around the consequences of mis-targetingSome ways to lower mis-targeting to non-diseased cells
BME 695N Lecture 2: Basic Concepts of Nanomedical Systems
28 Aug 2007 | Online Presentations | Contributor(s): James Leary
Outline: Features of NanomedicineBottoms up rather than top down approach to medicine Nano-tools on the scale of molecules Cell-by-cell repair approach – regenerative medicine Feedback control system to control drug dosing Elements of good engineering designWhenever possible, use a general...
BME 695N Lecture 1: Need for New Perspectives on Medicine
03 Aug 2007 | Online Presentations | Contributor(s): James Leary
Outline:The Progression of MedicineConventional "modern" medicine "Personalized" or "molecular" medicineNanomedicine "single-cell" medicineHow Conventional Medicine Works for Diagnosis of DiseaseIdentification of the "diseased state"Simple measurements of body structure and functionFollow-up...
BME 695N: Engineering Nanomedical Systems (Fall 2007)
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03 Aug 2007 | Courses | Contributor(s): James Leary
This course will cover the basic concepts of design of integrated nanomedical systems for diagnostics and therapeutics. Topics to be covered include: why nanomedical approaches are needed, cell targeting strategies, choice of core nanomaterials, technologies for testing composition and structure...
Nanotechnologies, Science and Society: Promises and Challenges
10 May 2007 | Online Presentations | Contributor(s): James Leary
Engineering Nanomedical Systems
06 Mar 2006 | Online Presentations | Contributor(s): James Leary
This tutorial discusses general problems and approaches to the design of engineered nanomedical systems. One example given is the engineering design of programmable multilayered nanoparticles (PMNP) to control a multi-sequence process of targeting to rare cells in-vivo, re-targeting to...
Nanofactories - In - Situ Production of Therapeutic Genes...
27 Jul 2005 | Online Presentations | Contributor(s): James Leary