BME 695L Lecture 10: Nanodelivery of Therapeutic Genes and Molecular Biosensor Feedback Control Systems

26 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 10.1      Introduction and overview 10.1.1    Some of the advantages of therapeutic genes 10.1.2    Some of the advantages of molecular biosensor feedback control …

BME 695L Lecture 11: Assessing Nanotoxicity at the Single Cell Level

27 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 11.1      Need for single cell measures of nanotoxicity 11.1.1    There is more than one way for a cell to die... 11.1.2    "Necrosis" vs. …

BME 695L Lecture 12: Assessing Drug Efficacy and Nanotoxicity at the Single Cell Level

22 Nov 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 12.1      Introduction to measures of efficacy for nanomedicine 12.1.1    for evaluation purposes, does structure/size reveal function? 12.1.2    nanomedical treatment at the …

BME 695L Lecture 13: Designing Nanomedical Systems (NMS) for In-vivo Use

22 Nov 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 13.1      Bringing in-vivo considerations into NMS design 13.1.1    the in-vitro to ex-vivo to in-vivo paradigm          13.1.1.1 In-vitro - importance of …

BME 695L Lecture 14: Designing and Testing Integrated Nanomedical Systems

22 Nov 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 14.1      Introduction to integrated designs 14.1.1    “Total design” but there is some order in the design process 14.1.2    A brief outline of the total design …

BME 695L Lecture 15: GMP and Issues of Quality Control Manufacture of Nanodelivery Systems

02 Dec 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 15.1      Overview 15.1.1    What does cGMP mean? 15.1.2    Why GMP? Controlling processes means more predictable …

BME 695L Lecture 16: FDA and EPA Regulatory Issues

02 Dec 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 16.1      Introduction and overview 16.1.1    How does the FDA think about nanomedical systems? 16.1.2    The 2006 Nanotechnology Task …

BME 695L Lecture 1: Need for New Perspectives on Medicine

31 Aug 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 1.1      Nanotechnology – Why is something so small so big? 1.1.1    Definitions of nanotechnology based on size 1.1.2    A “bottoms up” rather than “tops down” …

BME 695L Lecture 2: Designing Nanomedical Systems

31 Aug 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 2.1       Elements of good engineering design 2.1.1    Whenever possible, use a general design that has already been tested 2.1.2    Whenever possible, take advantage of …

BME 695L Lecture 3: Theranostics and Molecular Imaging

07 Sep 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 3.1      Nanomedical systems – levels of challenges 3.1.1    Diagnosis - difficult 3.1.2    Therapy – more difficult 3.1.3    Both ("Theragnosis") – most …

BME 695L Lecture 4: Cell Targeting and its Evaluation

03 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 4.1      Overview: targeting nanosystems to cells 4.1.1    antibody targeting 4.1.2    peptide targeting 4.1.3    aptamer …

BME 695L Lecture 5: Nanomaterials for Core Design

03 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 5.1      Introduction 5.1.1    core building blocks 5.1.2    functional cores 5.1.3    functionalizing the core …

BME 695L Lecture 6: Normal & Facilitated Cell Entry Mechanisms

04 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 6.1      Introduction 6.1.1    the general problem of cell entry 6.1.2    choosing modes of cell entry 6.1.3    how does Nature do it? …

BME 695L Lecture 7: Assessing Zeta Potentials

03 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 7.1      Introduction – the importance of the zeta potential 7.1.1    nanoparticle-nanoparticle interactions 7.1.2    nanoparticle-cell …

BME 695L Lecture 8: Surface Chemistry: attaching nanomedical structures to the core

12 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 8.1      Introduction 8.1.1    attachment strategies typically depend on core composition 8.1.2    but the attachment strategy should not drive the core …

BME 695L Lecture 9: Challenges of Proper Drug Dosing with Nanodelivery Systems

19 Oct 2011 | Online Presentations | Contributor(s): James Leary

See references below for related reading. 9.1      Overview of drug dosing problem 9.1.1    Problems of scaling up doses from animal systems 9.1.2    Basing dosing on size, area, weight of …

BME 695L: Engineering Nanomedical Systems

31 Aug 2011 | Courses | Contributor(s): James Leary

This course will cover the basic concepts of design of integrated nanomedical systems for diagnostics and therapeutics. Topics to be covered include: why nanomedical approaches are needed, cell targeting strategies, choice of core nanomaterials, technologies for testing composition and structure of …

BME 695N Lecture 10: Nanomaterials for core design

26 Sep 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction Core building blocks Functional cores Functionalizing the core surface Ferric oxide cores Paramagnetic cores Superparamagnetic cores Ferric nanorods Advantages and disadvantages C60 and carbon nanotubes Size and structure of C60 Elongation of C60 into …

BME 695N Lecture 13: Assessing Zeta Potentials

29 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction – the importance of the zeta potential Nanoparticle-nanoparticle interactions Nanoparticle-cell interactions Part of the initial nanomedical system-cell targeting process Low zeta potential leads to low serum protein binding and potentially longer circulation Zeta …

BME 695N Lecture 14: Challenges of proper drug dosing with nanodelivery systems

29 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Overview of drug dosing problem Problems of scaling up doses from animal systems Basing dosing on size, area, weight of recipient Vast differences between adults in terms of genetics, metabolism Dosing in children – children are NOT smaller adults! Pharmacokinetics – drug …

BME 695N Lecture 15: Nanodelivery of therapeutic genes & molecular biosensor feedback control systems

30 Oct 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction and overview Some of the advantages of therapeutic genes Some of the advantages of molecular biosensor feedback control systems Why a nanodelivery approach is appropriate The therapeutic gene approach What constitutes a "therapeutic gene" ? Transient versus stable …

BME 695N Lecture 16: Assessing drug efficacy at the single cell level

02 Nov 2007 | Online Presentations | Contributor(s): James Leary

Outline: Introduction and overview Nanomedical treatment at the single cell level requires evaluation at the single cell level For evaluation purposes, does structure reveal function? The difficulty of anything but simple functional assays The need for assays which at least show correlation to …

BME 695N Lecture 17: Assessing nanotoxicity at the single cell level

06 Nov 2007 | Online Presentations | Contributor(s): James Leary

Outline: Outline – the need for single cell measures of nanotoxicity There is more than one way for a cell to die... Necrosis" vs. "Apoptosis" There are other forms of "toxicity" Some other challenges in measuring toxicity of nanomaterials Necrosis vs. Apoptosis …

BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use

12 Nov 2007 | Online Presentations | Contributor(s): James Leary

Outline: Overview – the in-vitro to ex-vivo to in-vivo paradigm In-vitro - importance of choosing suitable cell lines Ex-vivo – adding the complexity of in-vivo background while keeping the simplicity of in-vitro In-vivo - all the complexity of ex-vivo plus the …

BME 695N Lecture 1: Need for New Perspectives on Medicine

03 Aug 2007 | Online Presentations | Contributor(s): James Leary

Outline: The Progression of Medicine Conventional "modern" medicine "Personalized" or "molecular" medicine Nanomedicine "single-cell" medicine How Conventional Medicine Works for Diagnosis of Disease Identification of the "diseased state" Simple measurements of body structure and …