KIST/PU Phage Display Selection of Tissue-Specific Homing Peptides and their Theragnostic Applications
23 Oct 2007 | Online Presentations | Contributor(s): Byung-Heon Lee
Several homing peptides that are specific to tumor cells, artherosclerotic plaques, stroke lesion, apoptotic cells and phosphatidylserine have been isolated by phage display in our laboratory. For example, the Bld-1 peptide is specific to bladder tumor cells and the AP-1 to atherosclerotic plaques. These peptides could be used in targeted delivery either alone or in combination with nanoparticles. We have demonstrated that the Bld-1 peptide can be homing to bladder tumor in vivo and showed a tumor specific PET imaging in rat and mouse models. Moreover, we assembled the Bld-1 peptide with hydrophobically-modified glycol chitosan (HGC) nanoparticles for carrying an anticancer drug and a nearinfrared optical imaging dye for targeted delivery and imaging simultaneously. The AP-1 peptide alone or assembled with HGC nanoparticles could home to atherosclerotic plaques in LDL receptor-deficient mice. These results demonstrate the potential of the homing peptide technology for affinity-based drug targeting and molecular imaging.