A Novel Diagnostic Assay Based On Nanomechanics
28 Jul 2005 | Online Presentations | Contributor(s): Marko Dorrestijn
Micro-fabricated silicon cantilevers arrays offer a novel label-free approach where ligand-receptor binding interactions occurring on the sensor generate nanomechanical signals like bending or a change in mass that is optically detected in-situ. We report the detection of multiple unlabelled biomolecules simultaneously down to picomolar concentrations within minutes. Differential measurements including reference cantilevers on an array of eight sensors enables sequence-specific detection of unlabelled DNA. It is suitable for meeting the ultimate challenge: label-free detection of specific RNA gene fragments within a complete genome will be shown (gene fishing).