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Applications of Atomic Force Microscopy (AFM) are ubiquitous in all bioscience areas that involve molecules (nucleic acids, lipids, proteins…), cells (living cells in culture, bacteria…), tissues (bone, cartilage, dentine, cornea…), polymers (biopolymers such as collagen) and other materials. This instrument allows high resolution imaging in 3 dimensions of samples in conditions close to their native state. It also permits force measurements such as protein folding or protein adhesion on the picoNewton scale.
During this presentation we will present the principles of AFM and demonstrate the applications in Life Sciences. We will also discuss key points about the technology, and more particularly, the technical characteristics you should understand when using an AFM such as noise level, limitations of the technology and tip artifacts.
Researchers should cite this work as follows:
Irene Revenko (2011), "Atomic Force Microscopy: Applications for Life Science Research," https://nanohub.org/resources/11017.
Digital Learning Center, Purdue University, West Lafayette, IN