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AFM Sample Preparation in Biology

By Irene Revenko

Asylum Research

Published on

Abstract

AFM is a powerful tool for imaging and force measurements for cells and biological samples. The key to excellent results is proper sample preparation. Biological sample preparation can appear simple but can easily impede your results. It is important to choose the right substrate to immobilize your sample, find the right way to attach the sample on the surface without modifying its structure, and still be able to work in an aqueous buffer. Since all biological samples are different, the key is to start with established recipes for similar samples, then adapt the procedures for a different one. We’ll present guidelines for choosing a substrate, immobilizing the sample, choosing the proper cantilever, as well as tips and tricks optimize AFM imaging and force measurements.

Bio

Irene Revenko Irene Revenko is an application scientist for Asylum Research since 2002. Prior to Asylum she was director of the Life Sciences Department at Veeco. Dr. Revenko received two Ph.D.s from Claude Bernard University, Lyon, France, one in 1998 on Applications of Atomic Force Microscopy in Biology and the other in 1994 on the Visualization of Type I Collagen Fibers.

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Researchers should cite this work as follows:

  • Irene Revenko (2011), "AFM Sample Preparation in Biology," http://nanohub.org/resources/11026.

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Digital Learning Center, Purdue University, West Lafayette, IN

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