See references below for related reading.
4.1 Overview: targeting nanosystems to cells
4.1.1 antibody targeting
4.1.2 peptide targeting
4.1.3 aptamer targeting
4.1.4 ligand-receptor targeting
4.2 Antibodies – polyclonal and monoclonal
4.2.1 Where do antibodies come from – in nature?
4.2.2 How do we make them in the laboratory?
4.2.3 Monoclonal antibodies – some details you need to know!
4.2.4 Labeling strategies
4.2.5 Therapy problems with mouse monoclonal antibodies
4.2.6 “Humanizing” monoclonal antibodies to reduce adverse host immune reactions
4.2.7 Why antibodies may not be a good overall choices for targeting nanosystems to cells
4.3 Peptide targeting
4.3.1 How does a peptide target?
4.3.2 Examples of peptide targeting
4.3.3 Creating new peptides by random peptide phage display libraries
4.3.4 High-throughput screening of those peptide libraries
4.3.5 Advantages and disadvantages of peptide targeting
4.4 Aptamer targeting
4.4.1 What are aptamers and how do they target?
4.4.2 Some different types of aptamers
4.4.3 How do you make aptamers?
4.4.4 How do you screen for useful aptamers?
4.5 Ligand-receptor targeting
4.5.1 What are ligands?
4.5.2 What are their advantages/disadvantages?
4.5.3 Example – folate receptors
4.6 How do we quantitatively evaluate targeting?
4.6.1 Technologies for evaluating targeting
18.104.22.168 Flow cytometry
22.214.171.124 Scanning image cytometry
4.6.2 Evaluating targeting specificity
4.6.3 Evaluating targeting sensitivity
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- McCarron,P.A., Marouf, W.M., Quinn,D.J., Fay,F., Burden,R.E., Olwill, S.A., Scott,C.J. "Antibody Targeting of Camptothecin-Loaded PLGA Nanoparticles to Tumor Cells". Bioconjugate Chem. 19, 1561–1569 (2008).
- Yang, X., Bassett, S.E., Li, X., Luxon, B.A., Herzog, N.K., Shope, R.E., Aronson, J., Prow, T.W., Leary, J.F., Kirby, R., Ellington, A.D., Gorenstein, D.G.: “Construction and selection of bead bound combinatorial oligonucleoside phosphorothioate and phosphorodithioate aptamer libraries designed for rapid PCR-based sequencing” Nucleic Acids Research 30 (23)e132: 1-8, 2003.
- Yang, X., Li, X., Prow, T.W. Reece, L.M., Bassett, S.E., Luxon, B.A., Herzog, N.K., Aronson, J., Shope, R.E., Leary, J.F., Gorenstein, D.G. “Immunofluorescence Assay and Flow Cytometric Selection of Bead Bound Aptamers” Nucleic Acids Research 31 (10): 1-8, 2003
- Ellington Lab Aptamer Database
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- Shapiro, H.M. Practical Flow Cytometry 4th edition 2003.
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1083 BME, Purdue University, West Lafayette, IN