BME 695L Lecture 6: Normal & Facilitated Cell Entry Mechanisms

By James Leary

Biomedical Engineering, Purdue University, West Lafayette, IN

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6.1      Introduction
6.1.1    the general problem of cell entry
6.1.2    choosing modes of cell entry
6.1.3    how does Nature do it? (biomimetics)

6.2      Non-specific uptake mechanisms
6.2.1    pinocytosis by all cells
6.2.2    phagocytosis by some cells
6.2.3    a recent study of Qdot NP uptake

6.3      Nanoparticle uptake
6.3.1    NP size matters
6.3.2    NP shape matters
6.3.3    NP agglomeration reduces uptake
6.3.4    A sample study

6.4      Receptor-mediated uptake
6.4.1    Receptor mediated transport of desired molecules
6.4.2    How to study uptake mechanisms by inhibiting pathways with specific drugs
6.4.3    Some sample studies

6.5      Effects of nanoparticle coatings
6.5.1    Acrylate-Facilitated Cellular Uptake
6.5.2    Polyethyleneimine Coating Enhancement of Cellular Uptake

6.6      Effects of microenvironment and external stimuli


Copyright © 2011, James F. Leary, All rights reserved.


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Xiao, Y., Forry, S.P., Gao, X., Holbrook, R.D., Telford, W.G., Tona, A. “Dynamics and mechanisms of quantum dot nanoparticle cellular uptake” Journal of Nanobiotechnology 8:13 pp. 1-9 (2010).
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Xia, T., Kovochich, M., Liong, M., Meng, H., Kabehie, S., George, S., Zink, J.I., Nel, A.E. “Polyethyleneimine Coating Enhances the Cellular Uptake of Mesoporous Silica Nanoparticles and Allows Safe Delivery of siRNA and DNA Constructs” ACS Nano 3(10): 3273–3286, 2009.
Krpetić, Ž., Nativo, P., Prior, I. A. and Brust, M. “Acrylate-Facilitated Cellular Uptake of Gold Nanoparticles” Small 7: 1982–1986, (2011).
Guo, S., and Huang, L. “Nanoparticles Escaping RES and Endosome: Challenges for siRNA Delivery for Cancer Therapy” Journal of Nanomaterials Volume 2011 (2011), Article ID 742895, 12 pages doi:10.1155/2011/742895
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