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BME 695L Lecture 10: Nanodelivery of Therapeutic Genes and Molecular Biosensor Feedback Control Systems

By James Leary

Biomedical Engineering, Purdue University, West Lafayette, IN

Published on

Abstract

See references below for related reading.

10.1      Introduction and overview
10.1.1    Some of the advantages of therapeutic genes
10.1.2    Some of the advantages of molecular biosensor feedback control systems
10.1.3    Why a nanodelivery approach is appropriate

10.2      The therapeutic gene approach
10.2.1    What constitutes a "therapeutic gene" ?
10.2.2    Transient versus stable expression modes

10.3      Molecular feedback control systems
10.3.1    Drug delivery has traditionally not used feedback controls
10.3.2    Why feedback control might be a very good idea!
10.3.3    Positive or negative feedback?

10.4      Molecular Biosensors as a component of a nanomedicine feedback control system
10.4.1    What is a molecular biosensor?
10.4.2    How a molecular biosensor functions as a therapeutic gene switch

10.5      Building integrated molecular biosensor/gene delivery systems –some examples
10.5.1    Example of a ribozyme/antivirus system
10.5.2    Example of an ARE biosensor/DNA repair system
10.5.3    Example of a feedback-controlled system for treatment of retinopathies

Credits

Copyright © 2011, James F. Leary, All rights reserved.

References

Fahmy, T.M., Fong, P.M., Park, J., Constable, T., Saltzman, W.M. “Nanosystems for Simultaneous Imaging and Drug Delivery to T Cells”. The AAPS Journal 2007; 9 (2).
Jabr-Milane, L., van Vlerken, L., Devalapally, H., Shenoy, D., Komareddy, S., Bhavsar, M., Amij, M. “Multi-functional nanocarriers for targeted delivery of drugs and genes” Journal of Controlled Release 100: 121–128 (2008). (Full text available at nih.gov)
Prow, T.W., Kotov, N.A., Lvov, Y.M., Rijnbrand, R., Leary, J.F. “Nanoparticles, Molecular Biosensors, and Multispectral Confocal Microscopy” Journal of Molecular Histology, 35(6): 555-564, 2004. (Full text available at nih.gov)
Prow, T.W., Salazar, J.H., Rose, W.A., Smith, J.N., Reece, L.M., Fontenot, A.A., Wang, N.A., Lloyd, R.S., Leary, J.F. "Nanomedicine – nanoparticles, molecular biosensors and targeted gene/drug delivery for combined single-cell diagnostics and therapeutics" Proceedings of SPIE 5318: 1-11, 2004.
Prow, T.W., Rose, W.A., Wang, N., Reece, L.M., Lvov, Y., Leary, J.F. "Biosensor-Controlled Gene Therapy/Drug Delivery with Nanoparticles for Nanomedicine" Proc. of SPIE 5692: 199 – 208, 2005.
Prow, T.W., Smith, J.N., Grebe, R., Salazar, J.H., Wang, N., Kotov, N., Lutty, G., Leary, J.F. "Construction, Gene Delivery, and Expression of DNA Tethered Nanoparticles" Molecular Vision 12: 606-615, 2006.
Prow, T.W., Grebe, R., Merges, C., Smith, J.N., McLeod, D.S., Leary, J.F., Gerard A.Lutty, G.A. "Novel therapeutic gene regulation by genetic biosensor tethered to magnetic nanoparticles for the detection and treatment of retinopathy of prematurity" Investigative Ophthalmology 2005;46: E-Abstract 1408.
Prow, T.W. “Nanomedicine – Targeted Nanoparticles for the delivery of Biosensors and Therapeutic Genes” PhD Thesis, University of Texas Medical Branch, January 2004. (Full text available at The University of Queensland)
Jin, S., Ye, K. “Nanoparticle-Mediated Drug Delivery and Gene Therapy” Biotechnology Progress 23: 32-41 (2007).
Groth, A.C., Olivares, E.C., Thyagarajan,B., Calos, M.P. “A phage integrase directs efficient site-specific integration in human cells” PNAS 97(11): 5995–6000 (2000).
Bertoni, C., Jarrahian, S., Wheeler, T.M., Li, Y., Olivares, E.C., Calos, M.P., Rando, T.A. “Enhancement of plasmid-mediated gene therapy for muscular dystrophy by directed plasmid integration” PNAS 103(2): 419–424 (2006).
Dobson, J. “Gene therapy progress and prospects: magnetic nanoparticle-based gene delivery” Gene Therapy 13: 283–287 (2006).

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Researchers should cite this work as follows:

  • James Leary (2011), "BME 695L Lecture 10: Nanodelivery of Therapeutic Genes and Molecular Biosensor Feedback Control Systems," http://nanohub.org/resources/12263.

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Location

1083 BME, Purdue University, West Lafayette, IN

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