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Illinois BioNanotechnology Seminar Series Fall 2011: Mechanotransduction at Cell-Cell Contact

By Hamid Tabdili

University of Illinois at Urbana-Champaign

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Abstract


Differential adhesion between cadherin subtypes expressed on cell surfaces is postulated to direct cell segregation during tissue morphogenesis. The studies described here used magnetic twisting cytometry and traction force microscopy to test the impact of cadherin binding selectivity on mechanotransduction and substrate rigidity sensing at cadherin-based adhesions. Micropipette measurements in turn quantified the binding affinities of different cadherin subtypes. Here we present evidence that the ability to transduce mechanical information across cadherin junctions depends on the identity of the ligand, such that only homophilic bonds between identical cadherins support force-activated junction remodeling. Mechanical stimulation with dissimilar cadherins or with anti-cadherin antibodies failed to elicit any response to force. Surprisingly, this behavior does not correlate with protein binding affinities, suggesting that mechanical differences may supercede protein-binding affinities in controlling intercellular organization during development.

Bio

Hamid Tabdili, Department of Chemical and Biomolecular Engineering

Credits

NCN@Illinois, Laura Miller, Omar Sobh, Zuhaib Sheikh, Nahil Sobh

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Cite this work

Researchers should cite this work as follows:

  • Hamid Tabdili (2012), "Illinois BioNanotechnology Seminar Series Fall 2011: Mechanotransduction at Cell-Cell Contact," http://nanohub.org/resources/12444.

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Time

Location

Beckman Institute, University of Illinois at Urbana-Champagn, Urbana, IL

Submitter

Zuhaib Bashir Sheikh

University of Illinois at Urbana-Champaign

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