[Illinois] Bioengineering Series: Non-conventional Raman Spectroscopy Applications Towards the Compositional Analysis of Soy Bean Seed
Raman spectroscopy has proven to be a non-destructive approach to obtaining chemically specific information from a wide variety of analytes. Conventional experimental configurations illuminate the sample and collect the backscattered Raman signal from the same points of illumination. However, by offsetting the illumination and collection regions, it is possible to tune the sampling depths to obtain signal from targets buried below the surface of light scattering materials. In this talk, a number of experimental examples will be presented illustrating applications for both spatially offset Raman spectroscopy and transmission Raman spectroscopy. We illustrate the ability to gain spectral/chemical information with both spatial and depth resolution. Employing two transmission instruments (built in-house), Raman spectra collected from soybeans were used to develop calibration models for protein, oil, amino acids, and fatty acids. Calibration curves using wet chemistry to evaluate the potential of Raman spectroscopy for the non-destructive compositional analysis of soybeans will be presented.
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