[Illinois] BioNanotechnology Seminar Series Fall 2012: DNAzymes as Novel Tools For Metal-Ion Sensing in Living Cells
Solely considered as a generic storage material, DNA was discovered to be capable of carrying out catalytic or enzymatic functions in 1990s. Since then, DNAzymes specific for a wide range of bioavailable metal ions have been selected through in vitro selection and have been converted into a large number of metal-ion specific sensors for environmental detection. Such a development has significantly expanded the number of metal ions one can detect. However, despite of such advancement, no report on using DNAzymes for cellular detection and actuation has been reported. In this talk, we will discuss the strategies and demonstrations for metal-ion sensing in living cells using DNAzymes. Nanoparticle-based DNAzyme probes, as well as photoactivatable DNAzymes have shown to be promising tools for intracellular metal-ion sensing.
Peiwen Wu is a graduate student in the department of Biochemistry. Her research goals are to detect and image the dynamics of metal ion distribution inside live cancer cells and correlate it with cell mechanical response. She is curious about how cell transduces mechanical signal to biochemical pathways. Peiwen earned her Bachelor's degree in Life Sciences at University of Science and Technology of China. Besides research, She likes running and swimming. Peiwen ran the Indianapolis half-marathon in Fall 2011.
Researchers should cite this work as follows:
Peiwen Wu (2012), "[Illinois] BioNanotechnology Seminar Series Fall 2012: DNAzymes as Novel Tools For Metal-Ion Sensing in Living Cells," https://nanohub.org/resources/16099.
MNTL 1000, University of Illinois Urbana Champaign, Urbana, IL