[Illinois] BioNanotechnology Seminar Series Fall 2012: Label-Free Electronic Detection of Cancer Biomarkers Using Silicon Nanowire Arrays

By Brian Dorvel

Biophysics, University of Illinois Urbana Champaign, Urbana, IL

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Abstract

Improving the performance and lowering the analyte detection limits of optical and electronic biosensors is essential for advancing wide ranging applications in diagnostics and drug discovery. One of these diagnostic platforms, based upon microfluidics coupled to ion-selective field effect transistors (ISFET's) offer great potential to address some world health goals since they can be made portable, low-cost, miniaturized, and sensitive. One of the biological targets of interest is miRNA, a small RNA which helps regulate protein expression, and its over or underexpression is commonly represented in multiple forms of cancer. Unfortunately, a number of performance issues, such as gate leakage and current instability due to fluid contact, have prevented widespread adoption of the technology for routine use. By using high-k dielectrics, such as hafnium oxide (HfO2), we have been able to address these challenges by passivating the exposed surfaces against destabilizing concerns of ion transport. With these fundamental stability issues addressed, we have created a fabrication process for HfO2 dielectric-based silicon nanowires for the detection of miRNA down to femtomolar levels.

Bio

Brian Dorvel, M-CNTC Trainee Brian is a PhD student in the Department of Biophysics at the University of Illinois at Urbana-Champaign Advisers: Rashid Bashir (ECE and BioE) and Susan Clare, Indiana University School of Medicine.

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Cite this work

Researchers should cite this work as follows:

  • Brian Dorvel (2012), "[Illinois] BioNanotechnology Seminar Series Fall 2012: Label-Free Electronic Detection of Cancer Biomarkers Using Silicon Nanowire Arrays," http://nanohub.org/resources/16100.

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Time

Location

MNTL 1000, University of Illinois at Urbana-Champaign, Urbana, IL

Submitter

NanoBio Node, Obaid Sarvana

University of Illinois at Urbana-Champaign

Tags

[Illinois] BioNanotechnology Seminar Series Fall 2012: Label-Free Electronic Detection of Cancer Biomarkers Using Silicon Nanowire Arrays
by: Brian Dorvel
  • Label-Free Electronic Detection of Cancer Biomarkers Using Silicon Nanowire Arrays 1. Label-Free Electronic Detectio… 0
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  • Outline 2. Outline 27.870967741935484
    00:00/00:00
  • What is an ISFET? 3. What is an ISFET? 45.744710440051648
    00:00/00:00
  • Ion Selective Field Effect Transistors 4. Ion Selective Field Effect Tra… 82.93553193602861
    00:00/00:00
  • Integrated Semiconductor Biosensor 5. Integrated Semiconductor Biose… 139.96145822985994
    00:00/00:00
  • Silicon Nanowires 6. Silicon Nanowires 197.35929273865102
    00:00/00:00
  • Outline 7. Outline 283.88993741929073
    00:00/00:00
  • Applications 8. Applications 286.86520313896892
    00:00/00:00
  • Breast Cancer Biomarkers: miRNA 9. Breast Cancer Biomarkers: miRN… 343.27128240786732
    00:00/00:00
  • Outline 10. Outline 389.63583987285188
    00:00/00:00
  • Device Stability : Examples 11. Device Stability : Examples 402.40468858647063
    00:00/00:00
  • HfO2 Chemical Optimization 12. HfO2 Chemical Optimization 521.29134796861024
    00:00/00:00
  • HfO2 Electrical Optimization 13. HfO2 Electrical Optimization 580.17681533724044
    00:00/00:00
  • Silicon HfO2 BioFET Device 14. Silicon HfO2 BioFET Device 610.05344193900862
    00:00/00:00
  • BioFET Device Performance 15. BioFET Device Performance 690.88149399026531
    00:00/00:00
  • Nanowire pH Measurements 16. Nanowire pH Measurements 722.61766166683219
    00:00/00:00
  • pH Sensitivity 17. pH Sensitivity 762.65977947750082
    00:00/00:00
  • Device Functionalization for miRNA detection 18. Device Functionalization for m… 783.36267011026132
    00:00/00:00
  • Poly-l-lysine Monolayer Deposition 19. Poly-l-lysine Monolayer Deposi… 824.89242078076882
    00:00/00:00
  • Probe Deposition Optimization 20. Probe Deposition Optimization 935.84503824376679
    00:00/00:00
  • Probe Conjugation 21. Probe Conjugation 970.80441044998508
    00:00/00:00
  • miRNA Sensing 22. miRNA Sensing 995.97019966226276
    00:00/00:00
  • Outline 23. Outline 1057.4590245356114
    00:00/00:00
  • Overall Goal: Monitoring Breast Cancer Therapy 24. Overall Goal: Monitoring Breas… 1063.7814641899276
    00:00/00:00
  • Future Work- Integrated Device 25. Future Work- Integrated Device 1102.7078573557167
    00:00/00:00
  • Questions and Comments? 26. Questions and Comments? 1137.4192907519619
    00:00/00:00