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[Illinois] ECE 416: Biosensors

By Brian Cunningham

University of Illinois at Urbana-Champaign

Category

Courses

Published on

Abstract

Learn the underlying engineering principles used to detect small molecules, DNA, proteins, and cells in the context of applications in diagnostic testing, pharmaceutical research, and environmental monitoring. Biosensor approaches including electrochemistry, fluorescence, acoustics, and optics will be taught. The course also teaches aspects of selective surface chemistry, including methods for biomolecule attachment to transducer surfaces. Students will learn how biosensor performance is characterized and will analyze case studies of commercial biosensor systems. Blood glucose detection, fluorescent DNA microarrays, label-free biochips, and bead-based assay methods will be covered. The course teaches classical methods for biodetection, but also extends into current areas of research and novel sensors involving nanotechnology, photonic crystals, and new tools used in the fields of genomics and proteomics.

To see the full list of lectures, please scroll down or click the 'View Course Lectures' button near the upper right of page.

Bio

My research group is focused on the application of sub-wavelength optical phenomena and fabrication methods to the development of novel devices and instrumentation for the life sciences. The group is highly interdisciplinary, with expertise in the areas of microfabrication, nanotechnology, computer simulation, instrumentation, molecular biology, and cell biology. In particular, we are working on biosensors based upon photonic crystal concepts that can either be built from low-cost flexible plastic materials, or integrated with semiconductor-based active devices, such as light sources and photodetectors, for high performance integrated detection systems.

Using a combination of micrometer-scale and nanometer-scale fabrication tools, we are devising novel methods and materials for producing electro-optic devices with nanometer-scale features that can be scaled for low-cost manufacturing. Many of our techniques are geared for compatibility with flexible plastic materials, leading to applications such as low cost disposable sensors, wearable sensors, flexible electronics, and flexible displays. Because our structures manipulate light at a scale that is smaller than an optical wavelength, we rely on computer simulation tools such as Rigorous Coupled Wave Analysis (RCWA) and Finite Difference Time Doman (FDTD) to model, design, and understand optical phenomena within photonic crystals and related devices.

In addition to fabricating devices, our group is also focused on the design, prototyping, and testing of biosensor instrumentation for high sensitivity, portability, and resolution. Advanced instruments enable high resolution imaging of biochemical and cellular interactions with the ability to monitor images of biochemical interactions as a function of time. Using the sensors and instrumentation, we are exploring new applications for optical biosensor technology including protein microarrays, biosensor/mass spectrometry systems, and microfluidics-based assays using nanoliter quantities of reagents. The methods and systems developed in the laboratory are applied in the fields of life science research, drug discovery, diagnostic testing, and environmental monitoring.

-From Professor Cunningham's Faculty Profile

Cite this work

Researchers should cite this work as follows:

  • Brian Cunningham (2013), "[Illinois] ECE 416: Biosensors," http://nanohub.org/resources/16705.

    BibTex | EndNote

Time

Location

University of Illinois, Urbana-Champaign, IL

Submitter

NanoBio Node, Obaid Sarvana, George Daley

University of Illinois at Urbana-Champaign

Tags

See also

Lecture Number/Topic Online Lecture Video Lecture Notes Supplemental Material Suggested Exercises
[Illinois] ECE 416 Applications II View HTML
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           In this lecture, ideas are presented for how biosensors may capture biomolecules. One of these ideas was the Anthrex Spore...

[Illinois] ECE 416 Bioselective Layers View HTML
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           In this lecture, the four different immobilization methods of capture molecules are discussed: Adsorption, Covalent Bonding,...

[Illinois] ECE 416 Bio Structure/Function I View HTML
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           In this lecture, we are given the structure of the protein, its function, role and potentials to the field of biosensors. The...

[Illinois] ECE 416 Bio Structure/Function II + Mass Transport I View HTML
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           In this lecture, the roles of antibodies and their structures are elaborated on from the last lecture. Then, there is a revision...

[Illinois] ECE 416 Assay Formats View HTML
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           In this lecture, assays, which is the most widely used technique to detect molecules on surfaces are discussed. In the direct...

[Illinois] ECE 416 Mass Transport II View HTML
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           In this lecture, we look at the mass transfer coefficient of association and dissociation rates of molecules on the biosensor and...

[Illinois] ECE 416 Electrochemical Sensors View HTML
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           In this lecture, we begin discussing the three types of electrochemical sensors: Potentiometric, Amperometric, and Conductometry....

[Illinois] ECE 416 Glucose Sensor I View HTML
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           In this lecture, we continue the discussion on the oxygen sensor. It starts with a review of the measurement of O2 concentration...

[Illinois] ECE 416 Glucose Sensor II View HTML
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           In this lecture, we looked at the three generations of the glucose sensor and the ways it has changed. The first generation was...

[Illinois] ECE 416 Acoustic Wave Sensors View HTML
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           In this lecture, we look at the concepts and theories behind the acoustic wave sensor. This sensor communicates through the...

[Illinois] ECE 416 SPR Sensors I View HTML
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           This lecture was an introduction to Surface Plasmon Resonance (SPR) Sensors. It started out with an explanation of Coulomb's Law...

[Illinois] ECE 416 SPR Sensors II View HTML
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           In this lecture, it is a continuation of the SPR Sensors discussed in the last one. We start off with the definition of a Surface...

[Illinois] ECE 416 SPR Sensors III View HTML
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           In this lecture, we continued the SPR Sensors discussion. We started off with the coupling condition being determined by the...

[Illinois] ECE 416 Optical Sensors I View HTML
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           In this lecture, we begin our discussion of Optical Biosensors. We start off by showing the general properties of optical...

[Illinois] ECE 416 Optical Sensors II View HTML
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           In this lecture, there is a review of the previous lecture of SPR Sensors along with Reflection Interference Spectroscopy. We...

[Illinois] ECE 416 Optical Sensors III View HTML
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           In this lecture, we finished our discussion over optical sensors. We started with discussing a photon moving between 2 mirrors...

[Illinois] ECE 416 Impedance Based Sensors I View HTML
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           In this lecture, we were introduced to the concept of a new sensor called the impedance-based Biosensor. Biomolecules are...

[Illinois] ECE 416 Impedance Based Sensors II View HTML
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           In this lecture, we start off with a light review of the nanopore sequencing method which is based upon having a carefully...

[Illinois] ECE 416 Lumerical Optical Simulation View HTML
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           In this lecture, we looked at lumerical optical simulation. The motivation to pursue this topic is because there is now software...

[Illinois] ECE 416 Fluorescence I View HTML
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           In this lecture, we discussed Fluorescence molecules and how they can be used for detection of other molecules. The molecules are...

[Illinois] ECE 416 Fluorescence II View HTML
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           In this lecture, we start with a revision of the common organic dyes and the photostability and quenching concepts. Quenching is...

[Illinois] ECE 416 Fluorescence III and Homogeneous Assays I View HTML
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           In this lecture, we continue the discussion of Fluorescence. Examples of results are shown from the fluorescent dyes and how they...

[Illinois] ECE 416 Homogeneous Assays II View HTML
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           In this lecture, we start with a review of the previous lecture and fluorescence polarization and dyes on homogeneous assays. The...

[Illinois] ECE 416 Fluorescent Beads View HTML
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           In this lecture, we start off talking about the luminex company and their new technology of fluorescent beads and the things it...

[Illinois] ECE 416 Avidin-Biotin and Surface Functionalization I View HTML
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           In this lecture, we start off by taking a look at X-Ray Crystallography and how it is used to understand how biomolecules binding...

[Illinois] ECE 416 Surface Functionalization II View HTML
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           In this lecture, we start off the discussion talking about the different functional groups. We see that the amines as bases and...

[Illinois] ECE 416 DNA Microarrays I View HTML
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           In this lecture, we started off with a revision of DNA with genes and gene expressions and how DNA sends messages. The problem...

[Illinois] ECE 416 DNA MicroArrays II View HTML
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           In this lecture, we start off with a revision of the previous lecture. The Microarray scanner is used to read the detection and...

[Illinois] ECE 416 Protein Microarrays I View HTML
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           In this lecture, we discussed the concepts of a Protein Microarray. The goal would be to be able to detect individual proteins. In...

[Illinois] ECE 416 Protein Microarrays II, Next Gen Sequencing I View HTML
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           In this lecture, we start off by finishing the lecture of protein microarrays. We conclude with the practical issues and problems...

[Illinois] ECE 416 Next Gen Sequencing II View HTML
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           In this lecture, we start off with a revision of the Illumina company and their DNA sequencing technology. After that we move on...

[Illinois] ECE 416 Surfance Enhanced Raman Spectroscopy I View HTML
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           In this lecture, we started with the concept of a surface enhanced raman spectroscopy. It was observed that it is a powerful...

[Illinois] ECE 416 Surface Enhanced Raman Spectroscopy II View HTML
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           In this lecture, we continued our discussion on Surface Enhanced Raman Spectroscopy (SERS). We started off with a revision of the...

[Illinois] ECE 416 Guest Speaker Dr. Sua Myong YouTube
           In this lecture, Single Molecule Studies of Rad51 and Srs2 will be discussed by Dr. Myong. We first look at the different ways to...

[Illinois] ECE 416 Nanoparticles View HTML
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           In this lecture, we discussed the concept of nanoparticles and quantum dots. A nanoparticle has a solid object size and can be...

[Illinois] ECE 416 Guest Speaker Dr. Greg Underhill YouTube
           In this lecture, Dr. Greg Underhill explains the relations of stem cell fate in correspondence to biosensors. The lecture starts...

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