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Nisin Diffusion Tool

By Andrew Blanchard1, Ting Lu1

1. University of Illinois at Urbana-Champaign

Simulate cell fluorescence based on nisin concentration

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Version 1.2a - published on 13 Aug 2014

doi:10.4231/D3H41JN4M cite this

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Abstract

Certain strains of bacteria produce an antimicrobial peptide known as nisin. The production of nisin is regulated by a set of genes, as shown in supporting documents, which involves a positive feedback loop. Thus, active nisin serves to activate its own production. When nisin concentrations reach a certain threshold, it induces cell death, thereby regulating the density of the bacterial population. By introducing a fluorescent protein which is under the control of a nisin-inducible promoter, we can visualize nisin concentrations in a bacterial population. High levels of nisin will induce cell death, medium levels will induce fluorescence, and low levels will yield negligible changes.

To visualize this phenomenon, we have constructed a tool on nanohub to simulate nisin diffusion and interaction with a bacterial population. The user selects from a list of predefined initial distributions of nisin in the chemical mask above the agar layer. The nisin then diffuses across the agar layer to the cell layer according to a three dimensional diffusion equation with degradation.

Cite this work

Researchers should cite this work as follows:

  • Andrew Blanchard; Ting Lu (2014), "Nisin Diffusion Tool," http://nanohub.org/resources/nisindiffusion. (DOI: 10.4231/D3H41JN4M).

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