[Illinois] Genetic Engineering for Nisin Diffusion and Tool Walkthrough

By Asha Kirchhoff

University of Illinois at Urbana-Champaign

Published on

Abstract

In this video Illinois Bioengineering student Asha Kirchhoff discusses the fundamentals behind the Biomedical Engineering Society's Nisin Diffusion experiment and setup for Engineering Open House. She also provides a walkthrough for the Nisin Diffusion tool hosted here at Nanohub.org.

To see the tool and learn more about Nisin Diffusion and genetic engineering, click here

Cite this work

Researchers should cite this work as follows:

  • Asha Kirchhoff (2013), "[Illinois] Genetic Engineering for Nisin Diffusion and Tool Walkthrough," http://nanohub.org/resources/18970.

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Submitter

NanoBio Node

University of Illinois at Urbana-Champaign

Tags

[Illinois] Genetic Engineering for Nisin Diffusion and Tool Walkthrough
  • Interactive Engineering Outreach with the NanoBIO Nisin Diffusion Model 1. Interactive Engineering Outrea… 0
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  • Nisin experimental design 2. Nisin experimental design 30.346529163894431
    00:00/00:00
  • Genetically engineered lac bacteria produce nisin in a positive feedback loop 3. Genetically engineered lac bac… 67.1935627081021
    00:00/00:00
  • Genetically engineered lac bacteria produce nisin in a positive feedback loop 4. Genetically engineered lac bac… 140.46182019977803
    00:00/00:00
  • Nisin feedback induces GFP expression in the lac bacteria 5. Nisin feedback induces GFP exp… 181.24905660377357
    00:00/00:00
  • The nisin and GFP pathways result in 2 different functional properties 6. The nisin and GFP pathways res… 229.84661487236403
    00:00/00:00
  • The antibacterial and signaling properties combine to create a natural bandpass filter for GFP expression 7. The antibacterial and signalin… 355.43163152053273
    00:00/00:00
  • The bandpass behavior is utilized in spatial patterning with the bacteria 8. The bandpass behavior is utili… 483.496115427303
    00:00/00:00
  • Diffusion of nisin through agar leads to expression of GFP by nisin-sensitive cells 9. Diffusion of nisin through aga… 530.9779134295228
    00:00/00:00
  • Tool Walkthrough 10. Tool Walkthrough 551.18557158712542
    00:00/00:00
  • The model of nisin diffusion is governed by a 3 dimensional diffusion equation 11. The model of nisin diffusion i… 724.12841287458377
    00:00/00:00
  • The model of bacteria fluorescence illuminates the multi-scale nature of this experiment 12. The model of bacteria fluoresc… 776.69311875693677
    00:00/00:00
  • The Nisin diffusion tool and experiment were used as an engineering outreach exhibit by UIUC BMES 13. The Nisin diffusion tool and e… 871.160821309656
    00:00/00:00
  • Modeling Nisin-Induced Bacterial Fluorescence 14. Modeling Nisin-Induced Bacteri… 946.04073251942293
    00:00/00:00
  • The Nisin diffusion tool was effective in engaging audience interest and demonstrating active diffusion 15. The Nisin diffusion tool was e… 1009.2671476137625
    00:00/00:00