[Illinois] Optics-based Biosensors for Diagnostics, Cell Imaging, Pharmaceutical Research, and Tissue Imaging

By Brian Cunningham

University of Illinois at Urbana-Champaign

Published on

Abstract


Bio

My [Dr. Cunningham's] research group is focused on the application of sub-wavelength optical phenomena and fabrication methods to the development of novel devices and instrumentation for the life sciences. The group is highly interdisciplinary, with expertise in the areas of microfabrication, nanotechnology, computer simulation, instrumentation, molecular biology, and cell biology. In particular, we are working on biosensors based upon photonic crystal concepts that can either be built from low-cost flexible plastic materials, or integrated with semiconductor-based active devices, such as light sources and photodetectors, for high performance integrated detection systems.

Using a combination of micrometer-scale and nanometer-scale fabrication tools, we are devising novel methods and materials for producing electro-optic devices with nanometer-scale features that can be scaled for low-cost manufacturing. Many of our techniques are geared for compatibility with flexible plastic materials, leading to applications such as low cost disposable sensors, wearable sensors, flexible electronics, and flexible displays. Because our structures manipulate light at a scale that is smaller than an optical wavelength, we rely on computer simulation tools such as Rigorous Coupled Wave Analysis (RCWA) and Finite Difference Time Doman (FDTD) to model, design, and understand optical phenomena within photonic crystals and related devices.

In addition to fabricating devices, our group is also focused on the design, prototyping, and testing of biosensor instrumentation for high sensitivity, portability, and resolution. Advanced instruments enable high resolution imaging of biochemical and cellular interactions with the ability to monitor images of biochemical interactions as a function of time. Using the sensors and instrumentation, we are exploring new applications for optical biosensor technology including protein microarrays, biosensor/mass spectrometry systems, and microfluidics-based assays using nanoliter quantities of reagents. The methods and systems developed in the laboratory are applied in the fields of life science research, drug discovery, diagnostic testing, and environmental monitoring.

Cite this work

Researchers should cite this work as follows:

  • Brian Cunningham (2013), "[Illinois] Optics-based Biosensors for Diagnostics, Cell Imaging, Pharmaceutical Research, and Tissue Imaging," http://nanohub.org/resources/19228.

    BibTex | EndNote

Time

Location

1000 MNTL, University of Illinois at Urbana-Champaign, Urbana, IL

Submitter

NanoBio Node

University of Illinois at Urbana-Champaign

Tags

[Illinois] Optics-based Biosensors for Diagnostics, Cell Imaging, Pharmaceutical Research, and Tissue Imaging
  • Friendly Photons: Optical Sensors in Life Sciences and Medicine 1. Friendly Photons: Optical Sens… 0
    00:00/00:00
  • Acknowledgement 2. Acknowledgement 41.526692074442934
    00:00/00:00
  • Analytes 3. Analytes 94.307957579919133
    00:00/00:00
  • External Cavity Laser Biosensors 4. External Cavity Laser Biosenso… 222.13705653467613
    00:00/00:00
  • One-dimensional Photonic Crystals 5. One-dimensional Photonic Cryst… 254.23031967650874
    00:00/00:00
  • Photonic Crystals for Label-Free Biosensing 6. Photonic Crystals for Label-Fr… 357.24153505760285
    00:00/00:00
  • External Cavity Laser Biosensor 7. External Cavity Laser Biosenso… 463.99243152513924
    00:00/00:00
  • Overlaid Spectrum 8. Overlaid Spectrum 550.75550115447743
    00:00/00:00
  • Small Molecule Binding Detection 9. Small Molecule Binding Detecti… 570.3353627832455
    00:00/00:00
  • Side-by-side dual modes in neighboring microplate wells 10. Side-by-side dual modes in nei… 641.32130922407873
    00:00/00:00
  • Two Wavelength Laser Spectrum 11. Two Wavelength Laser Spectrum 896.707530327306
    00:00/00:00
  • Environmental Fluctuation Test 12. Environmental Fluctuation Test 956.13446250095365
    00:00/00:00
  • Bulk Refractive Index Test 13. Bulk Refractive Index Test 977.2126649881742
    00:00/00:00
  • Dorzolamide binding to Carbonic anhydrase II 14. Dorzolamide binding to Carboni… 1036.2316319523918
    00:00/00:00
  • Collection of 35 compounds 15. Collection of 35 compounds 1074.784344243534
    00:00/00:00
  • Small-Scale Screening 16. Small-Scale Screening 1097.0864423590449
    00:00/00:00
  • Slide 17: Untitled 17. Slide 17: Untitled 1111.2292362859541
    00:00/00:00
  • Dose response 18. Dose response 1439.0972762645915
    00:00/00:00
  • Smartphone Biosensors for Health, Environment, and Food Safety 19. Smartphone Biosensors for Heal… 1475.4741741054397
    00:00/00:00
  • Grand Challenges in Food and Pharmaceutical Safety 20. Grand Challenges in Food and P… 1479.6218204013121
    00:00/00:00
  • Grand Challenges in Environmental Monitoring 21. Grand Challenges in Environmen… 1555.0953841458761
    00:00/00:00
  • Smartphone Biosensors 22. Smartphone Biosensors 1580.7971923399712
    00:00/00:00
  • Photonic Crystal Biosensor 23. Photonic Crystal Biosensor 1615.2702525368124
    00:00/00:00
  • Special Purpose Cradle: Utilize Phone Camera as a Spectrometer 24. Special Purpose Cradle: Utiliz… 1662.0502632181278
    00:00/00:00
  • Comparison to a $150K, 60 lb laboratory instrument 25. Comparison to a $150K, 60 lb l… 1748.9468528267337
    00:00/00:00
  • Smartphone Spectrometer 26. Smartphone Spectrometer 1779.0682841229877
    00:00/00:00
  • Smartphone Spectrometer 27. Smartphone Spectrometer 1834.8915236133364
    00:00/00:00
  • iPhone App 28. iPhone App 1885.2752269779508
    00:00/00:00
  • Measuring PC Resonant Wavelength Shifts 29. Measuring PC Resonant Waveleng… 1912.3369192034788
    00:00/00:00
  • First Biomolecular Assay IgG Detection 30. First Biomolecular Assay IgG D… 1930.151400015259
    00:00/00:00
  • Future Vision – Multimode Smartphone Biosensor 31. Future Vision – Multimode Sm… 1966.3923094529639
    00:00/00:00
  • IL6 ELISA run on a 96-well plate reader 32. IL6 ELISA run on a 96-well pla… 2134.26999313344
    00:00/00:00
  • Smartphone Biosensor: full spectral data available for analysis 33. Smartphone Biosensor: full spe… 2230.2822974486025
    00:00/00:00
  • IL6 ELISA Detected using Smartphone Spectrometer 34. IL6 ELISA Detected using Smart… 2280.3905941482681
    00:00/00:00
  • Limits of Detection Compared 35. Limits of Detection Compared 2305.0743760810478
    00:00/00:00
  • Future Applications 36. Future Applications 2331.4289703315885
    00:00/00:00
  • Photonic Crystal Enhanced Microscopy 37. Photonic Crystal Enhanced Micr… 2334.8753501400561
    00:00/00:00
  • Motivation 38. Motivation 2343.3944223107569
    00:00/00:00
  • PC Designed for Cell Imaging 39. PC Designed for Cell Imaging 2435.4079938705145
    00:00/00:00
  • PC Enhanced Microscope 40. PC Enhanced Microscope 2481.8187535734705
    00:00/00:00
  • Panc-1 Cells on Fibronectin-Coated PC 41. Panc-1 Cells on Fibronectin-Co… 2539.0849744463371
    00:00/00:00
  • Panc-1 Cells on Fibronectin-Coated PC 42. Panc-1 Cells on Fibronectin-Co… 2619.512694877506
    00:00/00:00
  • Dental Stem Cell Attachment Movie 43. Dental Stem Cell Attachment Mo… 2637.3108924329827
    00:00/00:00
  • PCEM for Single Nanoparticle Detection 44. PCEM for Single Nanoparticle D… 2749.2573255849707
    00:00/00:00
  • AFM-Printed Polymer Nanoparticles 45. AFM-Printed Polymer Nanopartic… 2786.6783940060263
    00:00/00:00
  • TiO2 Nanoparticles 46. TiO2 Nanoparticles 2886.948363549333
    00:00/00:00
  • Au Nanoparticles 47. Au Nanoparticles 2933.5115365512256
    00:00/00:00
  • Photonic Crystal enhanced fluorescence 48. Photonic Crystal enhanced fluo… 3247.162074956565
    00:00/00:00
  • Personalized Medicine 49. Personalized Medicine 3258.9250297500994
    00:00/00:00
  • Point of Care Diagnostics 50. Point of Care Diagnostics 3264.0828047921073
    00:00/00:00
  • Cancer biomarkers 51. Cancer biomarkers 3306.5697165245447
    00:00/00:00
  • Goal: High sensitivity, multiplexed biomarker detection in serum 52. Goal: High sensitivity, multip… 3351.3275662672895
    00:00/00:00
  • Breast Cancer Biomarker Panel Detected in Serum 53. Breast Cancer Biomarker Panel … 3380.363737900223
    00:00/00:00
  • Many other biomarkers… 54. Many other biomarkers… 3415.9414611661673
    00:00/00:00
  • How a Fluorophore Works 55. How a Fluorophore Works 3422.8156579087331
    00:00/00:00
  • Surface Photonic Crystals 56. Surface Photonic Crystals 3454.4787928834171
    00:00/00:00
  • Photonic Crystal Enhanced Fluorescence 57. Photonic Crystal Enhanced Fluo… 3457.9037543365507
    00:00/00:00
  • Silicon PC 58. Silicon PC 3594.7018054517562
    00:00/00:00
  • Silicon PC 59. Silicon PC 3628.5655877342419
    00:00/00:00
  • Line Scanning Detection Instrument 60. Line Scanning Detection Instru… 3649.3849279812166
    00:00/00:00
  • Separate resonances for exitation and extraction 61. Separate resonances for exitat… 3673.6796380090495
    00:00/00:00
  • 62. "Sandwich" Assay 3692.30948730414
    00:00/00:00
  • Fluorescent image comparison PC versus Glass 63. Fluorescent image comparison P… 3738.37591745559
    00:00/00:00
  • Fluorescent intensity comparison 64. Fluorescent intensity comparis… 3774.6634862163714
    00:00/00:00
  • Dose-Response Curves 65. Dose-Response Curves 3791.4974030830595
    00:00/00:00
  • Dose-Response Curves 66. Dose-Response Curves 3795.0703864857951
    00:00/00:00
  • Detection Limits <1 pg/ml 67. Detection Limits <1 pg/ml 3824.1050427151549
    00:00/00:00
  • Future plans: Microfluidic Chip for Blood Droplet Test 68. Future plans: Microfluidic Chi… 3853.6243240847439
    00:00/00:00
  • Automation of Assay 69. Automation of Assay 3874.4791227785881
    00:00/00:00
  • Conclusion 70. Conclusion 3882.398555797075
    00:00/00:00
  • Conclusion 71. Conclusion 3996.79747368421
    00:00/00:00