[Illinois] Phys550 Lecture 26:Third Generation Sequencing & Gene Expression Analysis Tools

By Taekjip Ha

University of Illinois at Urbana-Champaign

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Researchers should cite this work as follows:

  • Taekjip Ha (2014), "[Illinois] Phys550 Lecture 26:Third Generation Sequencing & Gene Expression Analysis Tools," https://nanohub.org/resources/20030.

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University of Illinois at Urbana-Champaign, Urbana, IL

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NanoBio Node

University of Illinois at Urbana-Champaign

[Illinois] Phys550 Lecture 26:Third Generation Sequencing & Gene Expression Analysis Tools
  • Next Generation DNA Sequencing Methods 1. Next Generation DNA Sequencing… 0
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  • Limitation of Next Generation DNA Sequencing Techniques 2. Limitation of Next Generation … 387.11518213723832
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  • Real-Time DNA Sequencing from Single Polymerase Molecules 3. Real-Time DNA Sequencing from … 559.58903446397778
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  • Fluorescence Microscopy with Low Background 4. Fluorescence Microscopy with L… 1165.7898272262398
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  • Zero-Mode Waveguide 5. Zero-Mode Waveguide 1260.8728554570487
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  • Real-Time DNA Sequencing from Singhle Polymerase Molecules 6. Real-Time DNA Sequencing from … 1433.6834699990923
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  • Four dNTPs: Two Laser Excitation 7. Four dNTPs: Two Laser Excitati… 1574.1385821053586
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  • Sequencing DNA by Measuring Ionic Current 8. Sequencing DNA by Measuring Io… 1812.28003873037
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  • Oxford Nanopore Technologies 9. Oxford Nanopore Technologies 1905.2556204423734
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  • Lecture Outline 10. Lecture Outline 2030.3387697055887
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  • Introducing Gene Expression Analysis 11. Introducing Gene Expression An… 2098.2729280764925
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  • Polymerase Chain Reaction (PCR) 12. Polymerase Chain Reaction (PCR… 2790.7550606674936
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  • Step 1 13. Step 1 2935.5490332536533
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  • Step 2 14. Step 2 2952.7805077309454
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  • Annealing Temperature is Critical 15. Annealing Temperature is Criti… 2990.5905776271597
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  • PCR Uses a Thermal Cycler 16. PCR Uses a Thermal Cycler 3048.7313080577323
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  • Thermal Cycling Temperature Profile for PCR 17. Thermal Cycling Temperature Pr… 3085.4256709734032
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  • Real Time PCR 18. Real Time PCR 3201.9550667191143
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  • mRNA Quantification: Northern 19. mRNA Quantification: Northern 3412.94765348422
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  • mRNA Quantification: Northern 20. mRNA Quantification: Northern 3514.10508638688
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  • DNA Microarray 21. DNA Microarray 3644.51883566825
    00:00/00:00
  • DNA Microarray 22. DNA Microarray 3985.4293019455959
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