Single Molecule Imaging in Live Cells

By Kenneth Ritchie

Department of Physics and Astronomy, Purdue University, West Lafayette, IN

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Abstract

Bacteria, such as Escherichia coli and Caulobacter crescentus, are the most studied and perhaps best-understood organisms in biology. The advances in understanding of living systems gained from these organisms are immense. Application of single-molecule techniques in bacteria have presented unique difficulties owing to their small size and highly curved form. The aim of this review is to show advances made in single-molecule imaging in bacteria over the past 10 years, and to look to the future where the combination of implementing such high-precision techniques in well-characterized and controllable model systems such as E. coli could lead to a greater understanding of fundamental biological questions inaccessible through classic ensemble methods.

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Cite this work

Researchers should cite this work as follows:

  • Kenneth Ritchie (2015), "Single Molecule Imaging in Live Cells," http://nanohub.org/resources/22684.

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Physics, Room 238, Purdue University, West Lafayette, IN

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Single Molecule Imaging in Live Cells
  • Single molecule imaging in live cells 1. Single molecule imaging in liv… 0
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  • Untitled: Slide 2 2. Untitled: Slide 2 144.41107774441107
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  • Outline 3. Outline 287.88788788788787
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  • Three cell types of interest 4. Three cell types of interest 354.22088755422089
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  • Tissue cell 5. Tissue cell 380.28028028028029
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  • The Fluid Mosaic Model of the plasma membrane 6. The Fluid Mosaic Model of the … 427.52752752752752
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  • Three different membrane architectures 7. Three different membrane archi… 530.93093093093091
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  • The E. coli outer envelope 8. The E. coli outer envelope 648.78211544878218
    00:00/00:00
  • Untitled: Slide 9 9. Untitled: Slide 9 714.61461461461465
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  • The labels we use 10. The labels we use 761.09442776109449
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  • Objective-type total internal reflection microscope 11. Objective-type total internal … 889.82315648982319
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  • Imaging single molecules 12. Imaging single molecules 957.05705705705714
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  • Particle position determination 13. Particle position determinatio… 1102.3690357023691
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  • Outline 14. Outline 1131.5315315315315
    00:00/00:00
  • Tissue cell plasma membrane 15. Tissue cell plasma membrane 1137.9379379379379
    00:00/00:00
  • Transferrin receptor on NRK cell 16. Transferrin receptor on NRK ce… 1147.7811144477812
    00:00/00:00
  • Takahiro Fujiwara, Akihiro Kusumi, Kyoto University 17. Takahiro Fujiwara, Akihiro Kus… 1218.6186186186187
    00:00/00:00
  • Analysis of Diffusion 18. Analysis of Diffusion 1302.6359693026361
    00:00/00:00
  • Membrane Skeleton Fence 19. Membrane Skeleton Fence 1430.3636970303637
    00:00/00:00
  • Anchored-Protein 20. Anchored-Protein "Picket" Mode… 1519.91991991992
    00:00/00:00
  • translational diffusion 21. translational diffusion 1623.9906573239907
    00:00/00:00
  • Oligomerization-Induced Trapping 22. Oligomerization-Induced Trappi… 1714.8148148148148
    00:00/00:00
  • Outline 23. Outline 1799.7997997997998
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  • RBC plasma membrane 24. RBC plasma membrane 1811.1444778111445
    00:00/00:00
  • Spectrin and band 3 25. Spectrin and band 3 1867.3673673673675
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  • DIDS Biotin linker 26. DIDS Biotin linker 2035.6690023356691
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  • Video of a quantum dot 27. Video of a quantum dot 2079.8465131798466
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  • Typical trajectories of quantum dot labeled band 3 28. Typical trajectories of quantu… 2107.1404738071406
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  • Red cell pathologies 29. Red cell pathologies 2175.4754754754754
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  • Affect of hereditary anemias on erythrocyte membranes 30. Affect of hereditary anemias o… 2345.9125792459126
    00:00/00:00
  • GC Kodippili et al. (2009) Blood 113, 6237-6245 31. GC Kodippili et al. (2009) Blo… 2441.9419419419419
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  • Dmicro 32. Dmicro 2504.7380714047381
    00:00/00:00
  • Outline 33. Outline 2648.7153820487156
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  • Why E. coli? 34. Why E. coli? 2663.9973306639972
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  • Outline 35. Outline 2679.6463129796466
    00:00/00:00
  • Diffusion of GFP in the E. coli cytoplasm 36. Diffusion of GFP in the E. col… 2699.6329662996332
    00:00/00:00
  • Diffusion of GFP in the E. coli cytoplasm 37. Diffusion of GFP in the E. col… 2785.9526192859526
    00:00/00:00
  • Diffusion of GFP in the E. coli cytoplasm 38. Diffusion of GFP in the E. col… 2817.7510844177514
    00:00/00:00
  • Diffusion of GFP in the E. coli cytoplasm 39. Diffusion of GFP in the E. col… 2857.8244911578245
    00:00/00:00
  • Diffusion of GFP in the E. coli cytoplasm 40. Diffusion of GFP in the E. col… 2910.8775442108777
    00:00/00:00
  • Outline 41. Outline 3041.3413413413414
    00:00/00:00