This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence, single-molecule fluorescence imaging, FRET, super-resolution fluorescence imaging, and atomic force microscopy and, if time permits, a unit on computational Visual Molecular Dynamics tools. The course is targeted to graduate students and advanced undergraduates from physics, biophysics, engineering, MCB, and chemistry. Enrollment is limited to 12 due to lab space, so you are encouraged to register early. Lectures and Labs will be held in Loomis Laboratory and the Institute for Genomic Biology.