MCW07 Silicon Based Nanopore Sensors for Detection of DNA Molecules

By Samir Iqbal1; Demir Akin1; Rashid Bashir2

1. Purdue University 2. University of Illinois at Urbana-Champaign

View Presentation (SWF)

Additional materials available (4)

Licensed under General Performance Usage.

Published on

Abstract

Solid-state nanopores have emerged as possible candidates for next-generation DNA sequencing devices. In this talk, we will review our recent work in development of solid-state nanopore channels that are selective towards single strand DNA (ssDNA). Nanopores functionalized with a 'probe' of hair-pin loop DNA can, under an applied electrical field, selectively transport short lengths of 'target' ssDNA that are complementary to the probe. Even a single base mismatch between the probe and the target results in longer translocation pulses and a significantly reduced number of translocation events. Our single molecule measurements allow us to separately measure the molecular flux and the pulse duration, providing a tool to gain fundamental insight into the channel-molecule interactions. The results can be explained in the conceptual framework of diffusive molecular transport with particle-channel interactions.

Cite this work

Researchers should cite this work as follows:

  • Samir Iqbal, Demir Akin, Rashid Bashir (2007), "MCW07 Silicon Based Nanopore Sensors for Detection of DNA Molecules," https://nanohub.org/resources/3079.

    BibTex | EndNote

Time

Location

Burton Morgan Building, Purdue University, West Lafayette, IN

Tags