BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo

By James Leary

Purdue University

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  1. Assessing nanomedical system (NMS) targeting at the single cell level
    1. Fluorescent labeling of NMSs
    2. First estimates of NMS binding by fluorescence microscopy
    3. Internal of external binding by confocal microscopy
    4. Single-cell image/confocal analysis
    5. Flow cytometric quantitation of NMS binding to specific cell types
  2. Image confocal analysis of NMS binding to single cells
    1. Ability to scan/locate cells of interest
    2. Photobleaching challenges
    3. Optical sectioning for 3D location of NMSs on/within cells
  3. A quick overview of flow cytometry
    1. Basic principles
    2. Capabilities and limitations
    3. Use for assessing specificity and sensitivity
  4. Rare-event analysis of NMS targeting to desired cells
    1. Basic concepts of rare-event analysis
    2. Strategies for rare cell detection
    3. More advanced flow cytometry for ultra-rare cell detection
    4. Examples of rare cell detection
    5. Rare cell sampling statistics


Cite this work

Researchers should cite this work as follows:

  • James Leary (2007), "BME 695N Lecture 6: Rare-event targeting of cells in-vitro and in-vivo,"

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