In this brief paper we describe some of our recent efforts to construct multi-component, multi-functional nanomedical systems for delivery of therapeutic genes. We first describe the general philosophy of our approach. Then we describe three specific aspects of the overall construction in simple examples. Finally we show the results of these efforts including the successful delivery and expression of genes as seen through reporter gene expression and fluorescent analysis of single treated cells.
For the previous 10 years Dr. Leary was a tenured Professor of Internal Medicine (Division of Infectious Diseases), Pathology, Biophysics, Microbiology & Immunology, and Human Biological Chemistry & Genetics as well as an Assistant Director of the Biomedical Engineering Center, an Affiliated Senior Scientist in the Sealy Centers for Molecular Sciences, Structural Biology, Cancer Cell Biology, Vaccine Development, and the Program in Bioinformatics. He also served as Assistant Vice President of Research for Advanced Technology. Prior to this position Dr. Leary was an Assistant and Associate Professor of Experimental Pathology at the University of Rochester Medical School.
Dr. Leary's research and teaching career as a professor spans more than 29 years. His original training includes an aerospace engineering degree from M.I.T., a masters degree in astrophysics, and a Ph.D. in Biophysics from Penn State University with a postdoctoral fellowship in Immunology & Cytometry at Los Alamos National Laboratory. His research has been funded by NIH for more than 25 years. He is the holder of 7 issued U.S. Patents, with 3 currently pending and is the author of numerous papers in the fields of high-throughput technologies, rare-event analysis methods, minimal residual disease monitoring, developmental immunology, cancer research, and nanomedicine. Dr. Leary has served on numerous national expert review panels for more than 20 years and is a frequently invited speaker at national and international scientific conferences. He was elected to the College of Fellows, American Institute for Medical and Biological Engineering in 2007.
Dr. Leary’s current funded research spans three general areas: (1) development of new high-throughput (flow cytometry, interactive molecular imaging) technologies for minimal residual disease monitoring, stem/progenitor and cancer stem cell isolation and manipulation, (2) detection of pathogens by hybrid microfluidic flow/imaging and (3) smart bionano-engineered systems for single-cell drug/gene delivery for regenerative nanomedicine.
As part of the research related to his talk at the 2007 KIST-Purdue Symposium, he has been developing multilayered, smart nanosystems containing cell targeting, entry facilitation, and localization molecules with molecular biosensors controlling delivery of therapeutic genes into single cells. These are the subject of a number of peer-reviewed publications and pending patents.
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