Gold nanorod molecular probes (GNrMPs) were designed and fabricated for multiplex identification of cell surface markers in HBECs. Cells were probed directly using dark field microscopy integrated with a spectral imager for simultaneous detection of up to three surface markers. The immunophenotype composition of these cell lines indicative of their metastasis potential was assessed using the GNrMPs. Due to the high multiplexing capacity of gold nanorods, the multiplexing capability of the GNrMP assay could possibly be expanded to 15 or greater. The technique has the potential to become an important tool for diagnosis and prognosis of breast and other cancers.
Their team has developed a range of spectroscopic and biosensor technologies for disease diagnostics and food security monitoring. Tracking protein interactions and monitoring and detecting the dynamic state of single molecule events in cells is addressed through techniques such as Fluorescnece Correlation Spectroscopy, FRET, Fluorescence lifetime imaging, enhanced single cell Raman spectroscopy , Plasmonic sensing, and Optical Trap.
He has published over 130 refereed research articles in areas covering thermodynamics and viscoelactic modeling of biological systems, spectroscopic methods, bio and nanomaterial sensors, and is a member of American Chemical Society, Institute of Biological Engineering, Biophysical Society, and American Association for clinical Chemistry.
Partial funding from the Oncological Science Center and IUPUI Walter center grant is acknowledged. This work is also supported partially by Susan G. Komen Foundation grant BCTR0601111.
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