1. Introduction and overview
1. Some of the advantages of therapeutic genes
2. Some of the advantages of molecular biosensor feedback control systems
3. Why a nanodelivery approach is appropriate
2. The therapeutic gene approach
1. What constitutes a "therapeutic gene" ?
2. Transient versus stable expression modes
3. Molecular feedback control systems
1. Drug delivery has traditionally not used feedback controls
2. Why feedback control might be a very good idea!
3. Positive or negative feedback?
4. Molecular Biosensors as a component of a nanomedicine feedback control system
1. What is a molecular biosensor?
2. How a molecular biosensor functions as a therapeutic gene switch
5. Building integrated molecular biosensor/gene delivery systems –some examples
1. Required components
2. Example of a ribozyme/antivirus system
3. Example of an ARE biosensor/DNA repair system

• Prow, T.W., Kotov, N.A., Lvov, Y.M., Rijnbrand, R., Leary, J.F. “Nanoparticles, Molecular Biosensors, and Multispectral Confocal Microscopy” Journal of Molecular Histology, Vol. 35, No.6, pp. 555-564, 2004.
• Prow,T.W., Salazar, J.H., Rose, W.A., Smith, J.N., Reece, L.M., Fontenot, A.A., Wang, N.A., Lloyd, R.S., Leary, J.F. "Nanomedicine – nanoparticles, molecular biosensors and targeted gene/drug delivery for combined single-cell diagnostics and therapeutics" Proc. of SPIE 5318: 1-11, 2004.
• Prow, T.W., Rose, W.A., Wang, N., Reece, L.M., Lvov, Y., Leary, J.F. "Biosensor-Controlled Gene Therapy/Drug Delivery with Nanoparticles for Nanomedicine" Proc. of SPIE 5692: 199 – 208, 2005.
• Prow, T.W., Smith, J.N., Grebe, R., Salazar, J.H., Wang, N., Kotov, N., Lutty, G., Leary, J.F. "Construction, Gene Delivery, and Expression of DNA Tethered Nanoparticles" Molecular Vision 12: 606-615, 2006
• Prow, T.W., Grebe, R., Merges, C., Smith, J.N., McLeod, D.S., Leary, J.F., Gerard A. Lutty, G.A. "Novel therapeutic gene regulation by genetic biosensor tethered to magnetic nanoparticles for the detection and treatment of retinopathy of prematurity" Molecular Vision 12: 616-625, 2006.
• Prow, T.W. “Nanomedicine – Targeted Nanoparticles for the delivery of Biosensors and Therapeutic Genes” PhD Thesis, University of Texas Medical Branch, January 2004.

Researchers should cite this work as follows:

• James Leary (2007), "BME 695N Lecture 15: Nanodelivery of therapeutic genes & molecular biosensor feedback control systems," http://nanohub.org/resources/3472.

1. biosensing 1
2. course lecture 1
3. DNA/Nucleic Acids 1
4. drug delivery 1
5. gene delivery 1
6. nanomedicine 1
7. nanoparticles 1