BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use

By James Leary

Basic Medical Sciences and Biomedical Engineering, Purdue University, West Lafayette, IN

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  1. Overview – the in-vitro to ex-vivo to in-vivo paradigm
    1. In-vitro - importance of choosing suitable cell lines
    2. Ex-vivo – adding the complexity of in-vivo background while keeping the simplicity of in-vitro
    3. In-vivo - all the complexity of ex-vivo plus the “active” components of a real animal
  2. In-vivo systems are open, “active” systems with multiple layers of complexity
    1. In-vitro and ex-vivo are mostly “closed” systems, but not absolutely
    2. What is an “open” system?
    3. Attempts to isolate open systems
  3. Layers of complexity of in-vivo systems
    1. Human cells in nude mice – a mixture of in-vitro and in-vivo
    2. “Model” small animal systems
    3. Bbetter model larger animal systems
  4. Examples of the in-vitro to in-vivo experimental pathway
    1. Kopelman group – multifunctional NPs for MRI and photodynamic therapy
    2. Langer group – aptamer-targeted NPs for cancer therapy in-vivo
    3. Leary group – peptide-guided NPs to human tumors in nude mice magnetic nanoparticles as MRI contrast agents in tissue phantoms


  • Kopelman, R., Koo, Y-E, Philbert, M., Moffatc, B.A., Reddy,G.R., McConville, P., Hall, D.E., Chenevert, T.L., Bhojanie, M.S., Buck, S.M., Rehemtulla, A., Ross, B.D. Multifunctional nanoparticle platforms for in vivo MRI enhancement and photodynamic therapy of a rat brain cancer. Journal of Magnetism and Magnetic Materials 293: 404–410, 2005.
  • Farokhzad, O.C., Cheng, J., Teply, B.J., Sherifi, I., Jon, S., Kantoff, P.W., Richie, J.P., Langer, R. Targeted nanoparticle-aptamer bioconjugates for cancer chemotherapy in vivo. PNAS 103(16), 6315–6320, 2006

Cite this work

Researchers should cite this work as follows:

  • James Leary (2007), "BME 695N Lecture 18: Designing nanodelivery systems for in-vivo use,"

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