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You are here: HomeResourcesCoursesIllinois Physics 498: Introduction to Biological PhysicsAbout

Illinois Physics 498: Introduction to Biological Physics

By Paul R Selvin

University of Illinois at Urbana-Champaign

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Lecture Number/Topic Online Lecture Video Lecture Notes Supplemental Material Suggested Exercises
Lecture 1: Introduction to Biophysics
Understanding biology using simple ideas from physics
Notes
Lecture 2: Central Dogma of Biology; Partition Function
Nucleic Acids, DNA,RNA, Cell size, Nucleotides, Boltzman factor, Partition function
Notes
Lecture 3: Nucleic Acids, RNA, and Proteins
Nucleic Acids, Proteins, DNA Dimensions and Stability, How to make a nucleotide
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Lecture 4 : Applications of DNA Technology: FISH, PCR, Forensics
FISH (Florescence In Situ Hybridization), Gene Arrays("Chips") can be made
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Lecture 5: Magnetic Traps & DNA
Introduction I
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Lecture 6: Magnetic Tweezers
Introduction II and Applications
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Lecture 7: Single-Molecule of ATPase
ATPase - How it produces ATP?
Notes
Lecture 8: Resolutions
X-ray diffraction (atomic resolution) Electron (Imaging) Microscopy (nm-scale) Visible (Imaging) Microscopy (nm - µm)
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Lecture 9: X-ray Structure and FIONA
Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion
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Lecture 10: Mutagenesis
Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)
Notes
Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)
Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability
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Lecture 12: Ultra-Resolution
SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization …
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Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM
Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation …
Notes Notes
Lecture 14: FRET and Helicase Activity
FRET: measuring conformational changes of (single) biomolecules, Unzipping mystery of helicases
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Lecture 15: Confocal and STED Microscopy
Confocal Detection, Energy Transfer, Confocal Microscopy, STED (Stimulated Emission Depletion),Improved resolution
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Lecture 16: Optical Traps - Part 1
First Optical Trap built, Reflection, Refraction, Brownian motionYann Chemla - Assistant Professor of Physics - University of Illinois at Champaign-Urbana
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Lecture 17: Diffusion - Part 1
Diffusion, Directed motors, Thermal motion, nerve synapse, Efficiency of Diffusion
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Lecture 18: Magnetotaxis
Biochemical Mechanisms for Magnetic Orientation in Animals, guest lecture Klaus Schulten.
Notes
Lecture 19: Optical Traps - Part2
Biological application of optical traps, High resolution optical trapping, Brownian noise
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Lecture 20: Diffusion - Part2
Diffusion and bacteria moving, power consumed by bacteria, Introduction to Reynolds number, Where Bacteria Live, How E. Coli move and swim,
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Lecture 21: Nerves
Ion Channels,Ionic current, Gating current, Digital Ion Channels, Structural studies, X-ray Crystallography
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Lecture 22: Conformational Changes in Ion Channels
Voltage dependence, Spontaneous shut-off, Nerve Impulse propagation, Structure Pore Domain, Voltage Sensor
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Lecture 23: Vision
Summary of Ion Channels, Vision , Visual System, The Eye, Structure of the Eye, Signal Processing, Diffraction and Pupil
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Lecture 24: The 4 Molecules of life
Atoms, Molecules, Macromolecules, you! Amino Acids, Sugars used as signals, Fatty Acids/Lipids
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