| Lecture 1: Introduction to Biophysics |
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Understanding biology using simple ideas from physics
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| Lecture 2: Central Dogma of Biology; Partition Function |
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Nucleic Acids, DNA,RNA, Cell size, Nucleotides, Boltzman factor, Partition function
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| Lecture 3: Nucleic Acids, RNA, and Proteins |
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Nucleic Acids, Proteins, DNA Dimensions and Stability, How to make a nucleotide
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| Lecture 4 : Applications of DNA Technology: FISH, PCR, Forensics |
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FISH (Florescence In Situ Hybridization), Gene Arrays("Chips") can be made
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| Lecture 5: Magnetic Traps & DNA |
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Introduction I
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| Lecture 6: Magnetic Tweezers |
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Introduction II and Applications
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| Lecture 7: Single-Molecule of ATPase |
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ATPase - How it produces ATP?
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| Lecture 8: Resolutions |
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X-ray diffraction (atomic resolution)
Electron (Imaging) Microscopy (nm-scale)
Visible (Imaging) Microscopy (nm - µm)
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| Lecture 9: X-ray Structure and FIONA |
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Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion
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| Lecture 10: Mutagenesis |
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Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)
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| Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy) |
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Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability
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| Lecture 12: Ultra-Resolution |
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SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization...
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| Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM |
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Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation...
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| Lecture 14: FRET and Helicase Activity |
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FRET: measuring conformational changes of (single) biomolecules, Unzipping mystery of helicases
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| Lecture 15: Confocal and STED Microscopy |
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Confocal Detection, Energy Transfer, Confocal Microscopy, STED (Stimulated Emission Depletion),Improved resolution
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| Lecture 16: Optical Traps - Part 1 |
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First Optical Trap built, Reflection, Refraction, Brownian motionYann Chemla - Assistant Professor of Physics - University of Illinois at Champaign-Urbana
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| Lecture 17: Diffusion - Part 1 |
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Diffusion, Directed motors, Thermal motion, nerve synapse, Efficiency of Diffusion
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| Lecture 18: Magnetotaxis |
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Biochemical Mechanisms for Magnetic Orientation in Animals, guest lecture Klaus Schulten.
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| Lecture 19: Optical Traps - Part2 |
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Biological application of optical traps, High resolution optical trapping, Brownian noise
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| Lecture 20: Diffusion - Part2 |
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Diffusion and bacteria moving, power consumed by bacteria, Introduction to Reynolds number, Where Bacteria Live, How E. Coli move and swim,
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| Lecture 21: Nerves |
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Ion Channels,Ionic current, Gating current, Digital Ion Channels, Structural studies, X-ray Crystallography
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| Lecture 22: Conformational Changes in Ion Channels |
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Voltage dependence, Spontaneous shut-off, Nerve Impulse propagation, Structure Pore Domain, Voltage Sensor
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| Lecture 23: Vision |
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Summary of Ion Channels, Vision , Visual System, The Eye, Structure of the Eye, Signal Processing, Diffraction and Pupil
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| Lecture 24: The 4 Molecules of life |
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Atoms, Molecules, Macromolecules, you!
Amino Acids, Sugars used as signals, Fatty Acids/Lipids
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