Rao’ lab has developed, using in vitro evolution methods, a number of thermostable proteins. These mutants have been characterized using various spectroscopies and X-ray diffraction. Protein stability is seldom characterized with respect to interaction with surfaces. This aspect is being investigated in the lab. Ion selective field effect transistor (ISFET) was used as platform to develop a triglyceride sensor using evolved enzymes. Rao’ lab is further interested in expanding this platform for other analytes. Plasmonic coupling in metal particles as a method to detect biomolecules is another interest pursued in his laboratory.
Centre for Cellular and Molecular Biology, Hyderabad, India