Sensitivity Amplification in Biosensors using Nanoparticles and Enzymatic Macromolecules

By Cagri Savran

Purdue University

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Abstract

We present non-invasive methods for improving the sensitivity of label-free biosensors that offer the advantage of rapid and real-time detection but suffer from relatively low sensitivity. We present detection of cancer markers using the Quartz Crystal Microbalance and demonstrate that 2 order-of-magnitude amplification in sensitivity can be achieved by forming sandwich or “piggy back” layers using antibodies and functionalized gold nanoparticles. We also discuss detection of aptazymes i.e. RNA-based enzymatic macromolecules and their potential as signal amplifying agents in biosensors.

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Researchers should cite this work as follows:

  • Cagri Savran (2005), "Sensitivity Amplification in Biosensors using Nanoparticles and Enzymatic Macromolecules," http://nanohub.org/resources/533.

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Location

Purdue University, West Lafayette, IN

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