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Self-Assembled Monolayer of DNA Microarrays

By Joanny Tam

Polytechnic University of Puerto Rico

Published on

Abstract

Faculty Advisor(s): Jeong, Lee, Janes

Conductivity experiments with DNA have already been performed. With this in mind, we experimented using the charge transport of DNA to receive signals about detection of similar DNA sequences of another species through the linking of complementary ssDNA on chips; subsequently, these methods can be used to prove whether certain genetic codes are present in a given specimen of DNA. Experiments about the linking between biothiolated DNA (ssDNA) attached to gold nanoparticles and immobilized DNA (complementary ssDNA) on gold surfaces were performed. Some considerations were taken into account: the addition of the spacer thiol 6-mercapto-1hexanol (MCH), the concentration of the buffer, and the interactions with Spermidine. We proved the linking of complementary ssDNA on chips by colorimetric differentiation; although we did the Atomic Force Microscopy (AFM), we could not distinguish with this method between nanoparticles attached to the gold surfaces and the topography of the gold surfaces. For the Colorimetric Differentiation method, we added StreptavidinHorseradish Peroxidase (STA-HRP) to the biothiolated DNA that reacted with the dye giving a blue coloration that confirmed the linking between the two complementary ssDNA. Conductivity measurements are still yet to be performed in order to prove the theoretical assumptions.

Cite this work

Researchers should cite this work as follows:

  • (2004), "Self-Assembled Monolayer of DNA Microarrays," http://nanohub.org/resources/747.

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Location

Fu Room, Potter Building, Room 234 <br /> Purdue University, West Lafayette, IN

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