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[Illinois] PHYS 598BP Lecture 7: More FIONA
14 Jun 2016 | Online Presentations | Contributor(s): Paul R Selvin
This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence...
[Illinois] PHYS 598BP Lecture 10: FIONA IV
03 Jun 2016 | Online Presentations | Contributor(s): Paul R Selvin
Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels
16 Apr 2010 | Online Presentations | Contributor(s): Paul R Selvin
The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual...
Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)
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15 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads
Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity
Single Molecule Photostability
Lecture 12: Ultra-Resolution
SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated...
Lecture 10: Mutagenesis
10 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)