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Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels
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16 Apr 2010 | Online Presentations | Contributor(s): Paul R Selvin
The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual …
Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)
11 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability
Lecture 12: Ultra-Resolution
SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization …
Lecture 10: Mutagenesis
07 Apr 2008 | Online Presentations | Contributor(s): Paul R Selvin
Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)
Illinois Physics 498: Introduction to Biological Physics
07 Apr 2008 | Courses | Contributor(s): Paul R Selvin
We will apply simple yet powerful ideas of physics to gain some understanding of biology. (What is the inertia of a bacteria and how does this affect its behavior?) We will begin with atoms, move …
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