Tags: Fluorescence Imaging with One-Nanometer Accuracy (FIONA)

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Description

Fluorescence Imaging with One-Nanometer Accuracy (FIONA) is a technique for localizing a single dye, or a single group of dyes, to within ~1-nm accuracy. This high degree of precision is achieved using total internal reflection fluorescence microscopy, deoxygenation agents, and a high quantum yield, low-noise detector.

For nanoHUB Simulation see: FIONA

Resources (1-13 of 13)

  1. Illinois iOptics Lecture 1: Super Accuracy and Super-Resolution of Molecular Motors and Ion Channels

    16 Apr 2010 | | Contributor(s):: Paul R Selvin

    The standard diffraction limit of light is about 250 nm, meaning that you cannot "resolve" objects closer than this distance. Despite this, we have come up with a method to measure individual biomolecules with 1.5 nm spatial localization in x-y plane and 1-500 msec temporal resolution,...

  2. Illinois Physics 498: Introduction to Biological Physics

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    We will apply simple yet powerful ideas of physics to gain some understanding of biology. (What is the inertia of a bacteria and how does this affect its behavior?) We will begin with atoms, move to molecules, then macromolecules, then cells, and finally whole systems. For example, how do we...

  3. Illinois Tool: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

    out of 5 stars 

    01 Nov 2010 | | Contributor(s):: Paul R Selvin, Raheem Syed, Nahil Sobh

    Fluorescence Imaging with One Nanometer Accuracy

  4. Lecture 10: Mutagenesis

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)

  5. Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

    out of 5 stars 

    11 Apr 2008 | | Contributor(s):: Paul R Selvin

    Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at heads Nanometer spatial localization, Second temporal resolution, Single Molecule sensitivity Single Molecule Photostability

  6. Lecture 12: Ultra-Resolution

    out of 5 stars 

    11 Apr 2008 | | Contributor(s):: Paul R Selvin

    SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization Microscopy), Enhancing Resolution

  7. Lecture 13: Enhancing Resolution - FIONA - SHREC - DOPI - PALM - STORM

    out of 5 stars 

    15 Apr 2008 | | Contributor(s):: Paul R Selvin

    Current Methods of obtaining higher resolution using: FIONA : Flouresence Imaging with One Nanometer Accuracy SHREC : Single molecule High Resolution Co-localization DOPI : Defocused Orientation Position Imaging

  8. Lecture 9: X-ray Structure and FIONA

    out of 5 stars 

    07 Apr 2008 | | Contributor(s):: Paul R Selvin

    Accuracy vs. Resolution Measuring atomic distances Biomolecular Motors: Intra- AND Extra-Cellular Motion

  9. [Illinois] ECE 564: Modern Light Microscopy

    out of 5 stars 

    14 Mar 2016 | | Contributor(s):: Gabriel Popescu

    Current research topics in modern light microscopy: optics principles (statistical optics, Gaussian optics, elastic light scattering, dynamic light scattering); traditional microscopy (bright field, dark field, DIC, phase contract, confocal, epi-fluorescence, confocal fluorescence); current...

  10. [Illinois] PHYS 598BP Lecture 10: FIONA IV

    out of 5 stars 

    03 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  11. [Illinois] PHYS 598BP Lecture 6: FIONA and STORM

    out of 5 stars 

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  12. [Illinois] PHYS 598BP Lecture 7: More FIONA

    out of 5 stars 

    13 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...

  13. [Illinois] Physics 598BP Lecture 9: FIONA III: GFPs

    out of 5 stars 

    06 Jun 2016 | | Contributor(s):: Paul R Selvin

    This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence,...