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Lecture 11: FIONA (Fluorescence Imaging with One Nanometer Accuracy)
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11 Apr 2008 | | Contributor(s):: Paul R Selvin
Fluorescence Imaging with One Nanometer Accuracy, Specificity to look at headsNanometer spatial localization, Second temporal resolution, Single Molecule sensitivitySingle Molecule Photostability
Lecture 12: Ultra-Resolution
SHREC (Single molecule High Resolution Co localization), SHRIMP (Super-High Resolution Imaging with Photobleaching), DOPI (Defocused Orientation Position Imaging), PALM (PhotoActivated Localization Microscopy), Enhancing Resolution
Lecture 10: Mutagenesis
07 Apr 2008 | | Contributor(s):: Paul R Selvin
Site-Directed Mutagenesis to Isolate and Mutate DNA (for FIONA)
Illinois Physics 498: Introduction to Biological Physics
We will apply simple yet powerfulideas of physics to gain some understanding of biology. (What is theinertia of a bacteria and how does this affect its behavior?) We willbegin with atoms, move to molecules, then macromolecules, then cells,and finally whole systems. For example, how do we see?...