3D CELL CULTURE
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3D3C Facility Access Training
3D3C Facility Access Training
In order to work in Birck and specifically in BRK2087 and BRK2087A&B, you will need to complete training for multi-user facility from Birck and training modules on the REM site (see below) before taking laboratory-specific training/check-in for BRK2087 and BRK2087A&B.
Birck specific training (https://nanohub.org/groups/bnc/training)
- BNC 105 – General Training
- BNC 150 – Fume Hood Training – prerequistite BNC 105
Purdue REM safety training (https://www.purdue.edu/ehps/rem/home/training.htm)
- Biological Safety Review
- Bloodborne Pathogens
- Handling Human Cell Lines
- Liquid nitrogen (Safe Handling and Use of)
3D3C specific training
Please read the safety rules below before starting your work in 3D3C and perform online training as indicated
and as it pertains to your needs (e.g., cell culture, LCM, etc.).
Safety rules in 3D3C facility
- Both Birck specific training and Purdue REM safety training must be completed prior to starting work in the 3D3C laboratory.
- No open-toe shoes; no food, drink or gum.
- Wear safety glasses or use appropriate eye shielding in the laboratory unless you are away from hazardous operations (REM CHP eye and face protection section 6.4.4).
- Wear your lab coat and gloves when you work in the laboratory. Do not touch anything in the laboratory without wearing gloves. Place your belongings and phone aside in an isolated area where they will not come in contact with cells or chemicals that you do not want to inadvertently carry out of the laboratory.
- Receive briefing for safety precautions from Dr. Kwok or appropriate staff designated by Dr. Kwok, before your first use a specific piece of equipment or before performing a new procedure enabled by 3D3C. (Examples: to use double-gloves, the fume hood, a mask, etc.; and how to discard items that you have used for your cell culture and experiments). Each task/area has its own rules that protect the equipment, other users and you.
- Label all chemicals and liquids with which you are working (medium, drugs, buffers, enzymes, etc.) with your initials, the contents (even water), and the date.
- Keep all chemicals and liquids in proper container and in proper storage location.
- Cover biohazard waste bin with the lid when not in use.
- Keep all liquids below eye-level of the shortest person working in the laboratory- here it is first shelf level in order to protect any user.
- Items that you use in the cell culture hoods (biosafety cabinets) must stay in the hoods and not come out to use in the main laboratory unless this is their final destination. Also to prevent risk of chemical and biological contamination, items used in the main laboratory must not be used in the cell culture hoods. Exceptions to this rule are cell cultures, medium and reagents, etc. used to treat cells that should be carried back and forth between their clean storage area and the hoods in a clean manner.
- Remove your gloves when you finish working in the hood and put on a clean pair.
- Remove your cell culture-designated lab coat and put on your main lab-coat when you are finished working in the hood; Store the lab coats in the designated areas for cell culture lab-coats or main laboratory lab-coats.
Remember, your safety level increases when you stay alert and notice your environment in the laboratory—the work area as well as the people working there.
3D3C training for use of the facility
- Online training: Any new user of the facility should review a short document on the basics of cell culture available online https://nanohub.org/resources/22645. This document includes pictures and movies that indicate processes used to minimize the risk of contamination of cells in culture. At the end of the document, there is a short quiz to ensure that major aspects of cell culture meant to reduce the risk of contaminating the area and thus, the cells of others as well as yours, are retained.
- In-person training: Following the online training, please meet with Dr. Shirisha Chittiboyina (or Dr. Kwok) to review the cell culture process in the rooms. The training is usually done by showing how to perform key procedures such as cell feeding and cell splitting and seeding, (and as necessary cell thawing and freezing). Then, the user performs each procedure in front of the trainer according to a timing set by the user and the trainer. The third time a procedure needs to be done, the user is on her/his own, but S/He can contact 3D3C staff if needed.
Dr. Chittiboyna is present in the facility or in office 2044 (X60225) from Mondays to Wednesdays (12-2pm), Thursdays (9-11 am) and Fridays (3:30-5:30 pm). (Email address: email@example.com; outside of the times indicated for her presence in Birck, she is in her main laboratory in Lynn Hall, phone 756-4967881 ) . Dr. Kwok is permanently located in Birck and can be contacted as needed (firstname.lastname@example.org; 765-494-6697).
Laser Capture Microdissection
To be updated
To be updated
Basics of 3D Cell Culture – Two Days Hands-On Training Course
Microscopic Analysis and Visualization of 3D Cultures -- Two Days Hands-On Training Course
1. Cell Culture Basics – Two Days Hands-on Training Course
September 22 & 23 2016
February 18 & 19 2016
March 24 & 25 2016
April 21 & 22 2016
May 19 & 20 2016
2. Basics of 3D Cell Culture – Two Days Hands-On Training Course
August 25 & 26 2016
June 16 & 17 2016
3. Microscopic Analysis and Visualization of 3D Cultures -- Two Days Hands-On Training Course
November 17 & 18 2016