This series of videos combined with accompanying PowerPoint file comprise introductory training for Cell Culture Basics. This learning unit is part of the training required for access to the Three Dimensional Cell Culture Core (3D3C) facility at Purdue University.
The videos provide some detailed demonstration of processes that help keep cell culture safe for all users as well as for the cultured cells during freezing, thawing and passaging the cells. Specific information is given regarding aseptic techniques while manipulating cells and culture related items, tips on labeling culture vessels and other items, methods for adding or removing medium from cell culture vessels and seeding cells in cell culture vessels. These training videos also highlight simple rules to dispose of biohazardous sharps and clean the system used to aspirate the medium from cell cultures.
How to use this training:
1. Download the PowerPoint file by clicking on the Download button.
2. View the PowerPoint presentation by stepping through the pages.
3. When reaching a video page, click on the either the YouTube link or the download link below the image.
4. After watching the video, return to the PowerPoint presentation to continue the training.
A transcript for the videos can be found in the notes area for each video slide or can be downloaded.