Nanotechnology Workforce Development
Laboratory Safety, Techniques and Activities
Laboratory Safety
Chemical Lab Safety Rules Learning Module
Chemical Lab Safety Rules provides most of the safety rules that you should follow when working in a lab that contains potentially hazardous materials. Many of these rules are common sense; however, you can forget to apply them at times. Applying safety rules at all times, ensures that any environment you work in will be a safe environment.
In addition to the rules listed in this unit, be sure to study the rules specific to the laboratory or facility in which you work.
Laboratory Techniques
Cell Culture Basics Training
Purdue University (2015) Powerpoint Presentation with embedded links to videos.
Taught by Sophie A. Lelièvre Shirisha ChittiboyinaTim Kwok
This series of videos combined with accompanying PowerPoint file comprise introductory training for Cell Culture Basics. This learning unit is part of the training required for access to the Three Dimensional Cell Culture Core (3D3C) facility at Purdue University, and is appropriate for anyone learning this technique.
The videos provide some detailed demonstration of processes that help keep cell culture safe for all users as well as for the cultured cells during freezing, thawing and passaging the cells. Specific information is given regarding aseptic techniques while manipulating cells and culture related items, tips on labeling culture vessels and other items, methods for adding or removing medium from cell culture vessels and seeding cells in cell culture vessels. These training videos also highlight simple rules to dispose of biohazardous sharps and clean the system used to aspirate the medium from cell cultures.
Lab Exercise: Atomic Layer Deposition
13 Jan 2017 | Teaching Materials | Contributor(s): NACK Network
The objective of this lab is to evaluate the atomic layer deposition (ALD) process. The ALD process will be used to deposit a transparent conducting oxide (TCO). Principles of resistance and resistivity will also be discussed.
Lab Exercise: Ellipsometry
18 Jan 2017 | Teaching Materials | Contributor(s): NACK Network
The objective of this lab is to evaluate the ellipsometer. Multiple ellipsometers will be presented and compared. The procedural operation and working mechanisms of the ellipsometer will both be discussed.
Lab Exercise: Fluorescent Tagging of Liposomes
21 Oct 2016 | Teaching Materials | Contributor(s): Terry Kuzma
This lab examines the liposome as a carrier of fluorescent tags used in cancer identification. The liposomes will be created from raw materials, tagged, extruded to a proper size, filtered, and characterized.
Lab Exercise: Profilometry
18 Jan 2017 | Teaching Materials | Contributor(s): NACK Network
The objective of this online lab is to evaluate the profilometer. Multiple profilometers will be presented and compared.
Laboratory Activities
Nanotechnology Principles Labs
A series of lab experiments designed to teach students of any age about nanotechnology principles.
The Nanotechnology Principles Labs include:
- Basic Principles
- Lithography
- Synthesis of nanoparticles
- Nanocrystalline Solar Cells
- Water Splitting (Fuel Cell)