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Atomic Force Microscopy: Applications for Life Science Research

By Irene Revenko

Asylum Research

Published on

Abstract

Atomic Force Microscopy: Applications for Life Science Research Applications of Atomic Force Microscopy (AFM) are ubiquitous in all bioscience areas that involve molecules (nucleic acids, lipids, proteins…), cells (living cells in culture, bacteria…), tissues (bone, cartilage, dentine, cornea…), polymers (biopolymers such as collagen) and other materials. This instrument allows high resolution imaging in 3 dimensions of samples in conditions close to their native state. It also permits force measurements such as protein folding or protein adhesion on the picoNewton scale.

During this presentation we will present the principles of AFM and demonstrate the applications in Life Sciences. We will also discuss key points about the technology, and more particularly, the technical characteristics you should understand when using an AFM such as noise level, limitations of the technology and tip artifacts.

Bio

Irene Revenko Irene Revenko is an application scientist for Asylum Research since 2002. Prior to Asylum she was director of the Life Sciences Department at Veeco. Dr. Revenko received two Ph.D.s from Claude Bernard University, Lyon, France, one in 1998 on Applications of Atomic Force Microscopy in Biology and the other in 1994 on the Visualization of Type I Collagen Fibers.

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Cite this work

Researchers should cite this work as follows:

  • Irene Revenko (2011), "Atomic Force Microscopy: Applications for Life Science Research," https://nanohub.org/resources/11017.

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Location

Digital Learning Center, Purdue University, West Lafayette, IN

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