Measuring the size and size distributions of nanoparticles, whether engineered or naturally occurring, is critical in understanding their behavior in a biological system. The size of nanoparticles engineered for dual therapy and diagnosis (“theranosis”) applications is important in predicting their biodistribution in targeted and non-targeted cells and tissues. The size of biological nanoparticles may aid in elucidating the mechanisms of key molecular pathways in complex diseases. This presentation will highlight the use of dynamic light scattering (DLS), which measures the hydrodynamic size of nanoparticles in suspension, in characterizing specific examples of engineered and natural nanoparticles. In terms of engineered nanoparticles, the role of nanoparticle size in developing a novel method for nanoparticle detection for biodistribution studies, “nanobarcoding,” will be presented. In terms of natural nanoparticles, the role of chylomicron size in investigating the effects of obesity on triglyceride metabolism will be shown.
Burton Morgan 121, Purdue University, West Lafayette, IN