[Illinois] ECE 416 Lecture 36: Raman Spectroscopy

By Guillermo Monroy1, Brian Cunningham1

1. University of Illinois at Urbana-Champaign

Published on

Abstract

Learn the underlying engineering principles used to detect small molecules, DNA, proteins, and cells in the context of applications in diagnostic testing, pharmaceutical research, and environmental monitoring. Biosensor approaches including electrochemistry, fluorescence, acoustics, and optics will be taught. The course also teaches aspects of selective surface chemistry, including methods for biomolecule attachment to transducer surfaces. Students will learn how biosensor performance is characterized and will analyze case studies of commercial biosensor systems. Blood glucose detection, fluorescent DNA microarrays, label-free biochips, and bead-based assay methods will be covered. The course teaches classical methods for biodetection, but also extends into current areas of research and novel sensors involving nanotechnology, photonic crystals, and new tools used in the fields of genomics and proteomics.

Bio

My research group is focused on the application of sub-wavelength optical phenomena and fabrication methods to the development of novel devices and instrumentation for the life sciences. The group is highly interdisciplinary, with expertise in the areas of microfabrication, nanotechnology, computer simulation, instrumentation, molecular biology, and cell biology. In particular, we are working on biosensors based upon photonic crystal concepts that can either be built from low-cost flexible plastic materials, or integrated with semiconductor-based active devices, such as light sources and photodetectors, for high performance integrated detection systems.

Using a combination of micrometer-scale and nanometer-scale fabrication tools, we are devising novel methods and materials for producing electro-optic devices with nanometer-scale features that can be scaled for low-cost manufacturing. Many of our techniques are geared for compatibility with flexible plastic materials, leading to applications such as low cost disposable sensors, wearable sensors, flexible electronics, and flexible displays. Because our structures manipulate light at a scale that is smaller than an optical wavelength, we rely on computer simulation tools such as Rigorous Coupled Wave Analysis (RCWA) and Finite Difference Time Doman (FDTD) to model, design, and understand optical phenomena within photonic crystals and related devices.

In addition to fabricating devices, our group is also focused on the design, prototyping, and testing of biosensor instrumentation for high sensitivity, portability, and resolution. Advanced instruments enable high resolution imaging of biochemical and cellular interactions with the ability to monitor images of biochemical interactions as a function of time. Using the sensors and instrumentation, we are exploring new applications for optical biosensor technology including protein microarrays, biosensor/mass spectrometry systems, and microfluidics-based assays using nanoliter quantities of reagents. The methods and systems developed in the laboratory are applied in the fields of life science research, drug discovery, diagnostic testing, and environmental monitoring. -From Professor Cunningham's Faculty Profile

Cite this work

Researchers should cite this work as follows:

  • Guillermo Monroy; Brian Cunningham (2013), "[Illinois] ECE 416 Lecture 36: Raman Spectroscopy," https://nanohub.org/resources/17705.

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Time

Location

University of Illinois, Urbana-Champaign, IL

Submitter

NanoBio Node

University of Illinois at Urbana-Champaign

[Illinois] ECE 416 Lecture 36: Raman Spectroscopy
  • Fluorescence Definition 1. Fluorescence Definition 0
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  • Fluorescence Requires Absorption 2. Fluorescence Requires Absorpti… 55.88493213359768
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  • Scattering Versus Absorption 3. Scattering Versus Absorption 64.960329569728415
    00:00/00:00
  • Fluorescence Definition 4. Fluorescence Definition 117.52364967958499
    00:00/00:00
  • Scattering Versus Absorption 5. Scattering Versus Absorption 117.77158986878243
    00:00/00:00
  • Scattering 6. Scattering 120.49893194995423
    00:00/00:00
  • Scattering and Virtual States 7. Scattering and Virtual States 157.31805004577359
    00:00/00:00
  • Two types of scattering 8. Two types of scattering 223.64205065608792
    00:00/00:00
  • Two types of scattering 9. Two types of scattering 287.36267927982908
    00:00/00:00
  • How to detect scattering 10. How to detect scattering 331.12412267317671
    00:00/00:00
  • Two varieties of Raman Scattering: Stokes and Anti-Stokes 11. Two varieties of Raman Scatter… 354.18256026853828
    00:00/00:00
  • Raman Spectroscopy 12. Raman Spectroscopy 440.58971620384494
    00:00/00:00
  • Raman Spectroscopy Absorption 13. Raman Spectroscopy Absorption 495.01258773268233
    00:00/00:00
  • Raman Spectroscopy 14. Raman Spectroscopy 540.509612450412
    00:00/00:00
  • Modes of Molecular Vibration - 2 atoms 15. Modes of Molecular Vibration -… 565.42760146475439
    00:00/00:00
  • Modes of Molecular Vibration - 3 atoms 16. Modes of Molecular Vibration -… 589.97368019530063
    00:00/00:00
  • Modes of Molecular Vibration 17. Modes of Molecular Vibration 638.94186756179431
    00:00/00:00
  • Models for Bigger Molecules 18. Models for Bigger Molecules 674.64525480622513
    00:00/00:00
  • Models for Functional Groups 19. Models for Functional Groups 686.91829417149836
    00:00/00:00
  • Raman Peaks for Common Functional Groups 20. Raman Peaks for Common Functio… 723.8613823619163
    00:00/00:00
  • Raman Spectra = Unique Molecular Signature 21. Raman Spectra = Unique Molecul… 755.96963686298443
    00:00/00:00
  • Raman Instrumentation & Measurements 22. Raman Instrumentation & Measur… 808.40898687824222
    00:00/00:00
  • Raman Instrumentation 23. Raman Instrumentation 863.079798596277
    00:00/00:00
  • Raman Instrumentation 24. Raman Instrumentation 914.65135794934383
    00:00/00:00
  • Raman Imaging 25. Raman Imaging 967.21467805920054
    00:00/00:00
  • Biological Applications 26. Biological Applications 1092.7963838877022
    00:00/00:00
  • Drug Manufacturing Quality Control 27. Drug Manufacturing Quality Con… 1137.1776777540433
    00:00/00:00
  • Raman spectra of oxygenated and deoxygenated hemoglobin 28. Raman spectra of oxygenated an… 1185.5260146475434
    00:00/00:00
  • Drug-DNA Interaction Analysis 29. Drug-DNA Interaction Analysis 1219.4938205675924
    00:00/00:00
  • E. Coli Bacteria Measurement 30. E. Coli Bacteria Measurement 1295.8593988404029
    00:00/00:00
  • Bacteria Measurement 31. Bacteria Measurement 1319.9095971925542
    00:00/00:00
  • Raman Spectra of a Rat's Eye Lens 32. Raman Spectra of a Rat's Eye L… 1361.5635489777235
    00:00/00:00
  • Raman Spectra of Human Eye Lens 33. Raman Spectra of Human Eye Len… 1392.3081324382056
    00:00/00:00
  • Analysis of Gallstones 34. Analysis of Gallstones 1426.0279981690571
    00:00/00:00
  • Cancer Diagnosis 35. Cancer Diagnosis 1450.5740768996034
    00:00/00:00
  • Cancer Diagnosis 36. Cancer Diagnosis 1493.8396399145558
    00:00/00:00
  • Sources 37. Sources 1580.9906164174549
    00:00/00:00