[Illinois] iOptics Series: Super Resolution Structured Illumination Microscopy (SR-SIM)

By Sivaguru Mayandi

University of Illinois at Urbana-Champaign

Published on


Structured illumination superresolution imaging technique breaks the diffraction limit of light by half in optical microscopy which was held over a century. Moiré fringes, which contain high frequency information transferred to low frequency band by projecting a known frequency pattern over the unknown high frequency sample was exploited in this technique. By creating Moiré fringes using a known patterned illumination over the samples information beyond the conventional field of view is transferred into the actual viewable field. This high frequency information is computed using a set of sophisticated Fast Fourier Transformation (FFT) algorithms and the resolution of the optical system is improved as well as doubled. Cellular structures and proteins now could be visualized up to 120 nm in XY and 250 nm in Z axis. The improved resolution is handy for confirming co-localization studies of protein-protein interactions and accurate description of 3D volumes of biological specimens. The IGB Microscopy Facility is housing a Zeiss Elyra SR-SIM system available for the entire campus.


Dr. Sivaguru Mayandi has received his Masters from the American College (1989), PhD from the Madurai Kamaraj University in the area of Ecophysiology and Abiotic Stress (1995), and awarded a postdoctoral fellowship from the German Academic Exchange (DAAD) at the University of Hannover and Bonn, Germany (1995-1998), and was a postdoctoral fellow of the Japanese Society for Promotion of Science (JSPS) at the University of Okayama, where he received his second PhD in Plant Physiology (1998-2000), moved to University of Missouri at Columbia as a postdoctoral fellow and after few years (2000-2002) served as the Associate Director of the UMC Microscopy Core Facility (2002-2006), now serving as Assistant Director of the Microscopy Facility at the IGB-UIUC (2006-present). Source (https://publish.illinois.edu/spie-uofi/2013/03/29/super-resolution-structured-illumination-microscopy-sr-sim-march-26-4-5-pm-in-beckman-room-2269/)


The series includes lectures from graduate students, post-docs and faculty working at the cutting edge of optics and photonics research. The lectures will be held every Monday at Beckman Institute, Room 2269, from 12:00 to 1:00 pm.

We invite potential speakers to submit an abstract and preferred dates for the Spring semester. We particularly encourage senior graduate students and postdocs to use iOptics as an avenue to showcase their research.

Participation in the iOptics series is free and open to all faculty, staff, postdoctoral, doctoral and undergraduate students involved or interested in optics, photonics, and nano/bio photonics. Each talk is followed by a free lunch.

Please send your abstract or any other questions related to the symposium to: illinois.optics@gmail.com

Sponsored by

iOptics is a graduate student organized seminar on optics and photonics which is initiated by Prof. Popescu and his graduate students. The first seminar series started in fall 2009. It is currently sponsored by International Society for Optics and Photonics (SPIE).

Cite this work

Researchers should cite this work as follows:

  • Sivaguru Mayandi (2013), "[Illinois] iOptics Series: Super Resolution Structured Illumination Microscopy (SR-SIM)," https://nanohub.org/resources/18322.

    BibTex | EndNote



Beckman Room 2269, University of Illinois, Urbana-Champaign, IL


NanoBio Node, Obaid Sarvana

University of Illinois at Urbana-Champaign