Sequencing a Genome by a Torrent of Ions: How an Old pH-Meter Got Its Groove Back
Sequencing a Genome by a Torrent of Ions: How an Old pH-Meter Got Its Groove Back
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1. Sequencing a Genome by a Torre…
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2. Grand Challenges of Engineerin…
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3. Convergence of Disciplines
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4. Back-story: A Century-long Con…
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5. Outline
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6. The book of life written in th…
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7. Lab on a chip: pH-based genome…
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8. Conventional Sequence-by-Synth…
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9. DNA Synthesis releases Proton
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10. ISFET: Theory of pH-sensing
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11. Sequencing by Synthesis
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12. pH-based Genome Sequencers
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13. Modeling Approaches (Go, JAP, …
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14. Model Validation
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15. Outline
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16. Idealized Polymerase Chain Rea…
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17. How PCR Doubles DNA Count
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18. Emulsion PCR and Poisson Stati…
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19. Multiply Loaded Beads must be …
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20. How to avoid multiple loading
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21. Loading with Reference Wells
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22. Recall: Sequencing by synthesi…
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23. Homo-polymer Error (~3% for N=…
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24. Scaling challenges of Sequenci…
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25. Properties of Signal and Noise…
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26. Putting together the pieces Co…
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27. Droplet PCR: Multifunctional T…
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28. Promise of nanobiotechnology
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29. Implanted Sensors: Heart
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30. Sensing and actuation: Optogen…
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31. Conclusions
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