[Illinois] Physics 598BP: Experimental Biophysics
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Abstract
This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence spectroscopy, introduction to various microscope techniques - wide field, bright field, DIC, fluorescence, single-molecule fluorescence imaging, FRET, super-resolution fluorescence imaging, and atomic force microscopy and, if time permits, a unit on computational Visual Molecular Dynamics tools. The course is targeted to graduate students and advanced undergraduates from physics, biophysics, engineering, MCB, and chemistry. Enrollment is limited to 12 due to lab space, so you are encouraged to register early. Lectures and Labs will be held in Loomis Laboratory and the Institute for Genomic Biology.
Bio
Paul Selvin earned a Ph.D. from the University of California, Berkeley, in 1990. Formally it was in physics, but in reality, it was in biophysics. The last experiment for his thesis, for example, was measuring the torsional rigidity of DNA by looking at a fluorophore intercalated in DNA, twisting around. A paper based on this experiment was published in Science (Selvin, 1992, Science) and later confirmed in a single molecule experiment (Bryant, Nature, 2003).
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University of Illinois at Urbana-Champaign
Tags
Lecture Number/Topic | Online Lecture | Video | Lecture Notes | Supplemental Material | Suggested Exercises |
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[Illinois] PHYS 598BP Lecture 1: Welcome and Introduction to Lab 2 | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 2: Fluorescence, Lifetimes and FRET (Lab 1) | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 3: FRET | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 4: More FRET | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 5: Still more FRET | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 6: FIONA and STORM | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 7: More FIONA | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 8: smFRET | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] Physics 598BP Lecture 9: FIONA III: GFPs | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 10: FIONA IV | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |
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[Illinois] PHYS 598BP Lecture 11: 3D resolution in single molecule fluorescence | YouTube |
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This course provides training in advanced biophysical techniques through hands-on laboratory exercises and weekly lectures as background. Topics will cover general optical and fluorescence... |