3D Cell Culture Basics: Forming Spheroids in the Presence of Extracellular Matrix

Sophie A. Lelièvre; Shirisha Chittiboyina; Tim Kwok

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3D Cell Culture Basics: Forming Spheroids in the Presence of Extracellular Matrix

By Sophie A. Lelièvre¹; Shirisha Chittiboyina²; Tim Kwok³

1. Basic Medical Sciences and Cancer Pharmacology, Purdue University, West Lafayette, IN 2. Basic Medical Sciences, Purdue University, West Lafayette, IN 3. 3D3C Cell Culture Facility, Purdue University, West Lafayette, IN

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Supporting Docs

(PDF, 787.52 KB)3D Cell Culture Basics Training (download this first)
(PPTX, 1.83 MB)3D Cell Culture Basics Training PowerPoint (download this first)
(MP4, 73.45 MB)01 Coating Culture Vessels with EHS-derived Gel (e.g., Matrigel)
(MP4, 36.50 MB)02 Adding Cells with the 3D Embedding Culture Method (example with EHS-derived gel (e.g., Matrigel))
(MP4, 22.44 MB)03 Adding Cells with the 3D Drip Culture Method
(MP4, 30.48 MB)04 Adding the EHS-gel Derived Drip to the 3D Drip Cell Culture
(MP4, 14.17 MB)05 Adding Medium to a 3D Cell Culture Vessel Containing a Gel
(MP4, 21.39 MB)06 Removing Medium from a 3D Cell Culture Vessel Containing a Gel
(MP4, 17.81 MB)07 Dislodging cells with Dispase from Culture with EHS-derived Gel (e.g. Matrigel)