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BME 695N Lecture 17: Assessing nanotoxicity at the single cell level

By James Leary

Basic Medical Sciences and Biomedical Engineering, Purdue University, West Lafayette, IN

Published on

Abstract

Outline:
  1. Outline – the need for single cell measures of nanotoxicity
    1. There is more than one way for a cell to die...
    2. Necrosis" vs. "Apoptosis"
    3. There are other forms of "toxicity"
    4. Some other challenges in measuring toxicity of nanomaterials
  2. Necrosis vs. Apoptosis mechanisms
    1. Necrosis is unplanned "cell injury"
    2. Apoptosis is planned "programmed cell death"
    3. Why it is important to distinguish between necrosis and apoptosis
  3. Single cell assays for necrosis and apoptosis
    1. Dye exclusion assays for necrosis
    2. TUNEL assays for late apoptosis
    3. Annexin V assays for early apoptosis
    4. COMET assays for DNA damage and repair
    5. Light scatter assays
  4. Nanotoxicity in vivo – some additional challenges
    1. Single cell nanotoxicity, plus....
    2. Accumulations of nanoparticles can change toxicity locally to tissues and organs
    3. Filtration issues of nanoparticles – size matters – toxicity to liver and lung

References

Cite this work

Researchers should cite this work as follows:

  • James Leary (2007), "BME 695N Lecture 17: Assessing nanotoxicity at the single cell level," https://nanohub.org/resources/3497.

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Time

Location

Biomedical Engineering Building, Room 1083

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