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2010 Nano-Biophotonics Summer School @ UIUC Lecture 3 - Fourier optics - Nonlinear optics - Microscopy

By Kimani C Toussaint

Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL

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Ray optics-Limit of wave optics when wavelength is
infinitesimally small

Wave optics-provides a description of optical
phenomena using scalar wave theory

Electromagnetic optics- provides most complete
treatment of light within classical optics

Quantum optics-provides a quantum mechanical
description of the electromagnetic theory

Ernst Abbe explained effect of diffraction on
resolution (1873)
� Lord Rayleigh (John Strutt) established criterion
for optimum resolution from diffraction-limited
microscope (1879)
� Assumptions:
� Observations occur for conventional geometry (single
objective lens)
� Excitation light is uniform throughout the sample
� Linear absorption process (e.g., linear fluorescence)


Edited and Uploaded by Omar Sobh, University of Illinois at Urbana-Champaign



University of Illinois at Urbana-Champaign, Urbana, IL

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