Support

Support Options

Submit a Support Ticket

 

Illinois Tool: FIONA (Fluorescence Imaging with One Nanometer Accuracy)

By Paul R Selvin1, Raheem Syed1, Nahil Sobh1

1. University of Illinois at Urbana-Champaign

Fluorescence Imaging with One Nanometer Accuracy

Launch Tool

You must login before you can run this tool.

Version 1.3 - published on 31 Jan 2011

doi:10.4231/D3WS8HK8V cite this

This tool is closed source.

View All Supporting Documents

See also

No results found.

Category

Tools

Published on

Abstract

The standard diffraction limit of light is about 250 nm, meaning that you cannot “resolve” objects closer than this distance. Despite this, we have come up with a method to measure 1.5 nm in x-y plane, with 1-500 msec, using a technique we call Fluorescence imaging with one-nanometer accuracy (FIONA) is a technique for localization a single dye, or a single group of dyes, to within approximately 1-nm accuracy. This high degree of precision is achieved using total internal reflection fluorescence microscopy, deoxygenation agents, and a high quantum yield, low-noise detector.

“In Vitro and in Vivo FIONA and other Acronyms for Watching Molecular Motors Walk”, Paul R. Selvin, Tyler Lougheed, Melinda Tonks Hoffman, Hyokeun Park, Hamza Balci, Benjamin H. Belehm, and Erdal Toprak , Biophysics Center and Physics Department, University of Illinois, Urbana, Illinois 61801

Cite this work

Researchers should cite this work as follows:

  • Paul R Selvin; Raheem Syed; Nahil Sobh (2011), "Illinois Tool: FIONA (Fluorescence Imaging with One Nanometer Accuracy)," https://nanohub.org/resources/fiona. (DOI: 10.4231/D3WS8HK8V).

    BibTex | EndNote

Tags

nanoHUB.org, a resource for nanoscience and nanotechnology, is supported by the National Science Foundation and other funding agencies. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.